Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Nt PLC3 expression pattern (Northern analysis: 3' UTR probe)
• Supplemental Figure 2 - Phylogenetic analysis of pollen and Arabdiopsis PI PLCs.
• Supplemental Figure 3 - Intracellular localization of inactive Nt PLC3 mutants, and of wild type Nt PLC3 after blocking pollen tube growth with Latrunculin B.
• Supplemental Figure 4 - Normal PI 4,5-₂ and DAG distributions in pollen tubes treated with U-73122 at a low concentration, or with an inactive analog of this drug (U-73343).
• Supplemental Figure 5 - Co-localization of Nt-PLC3 and DAG in growing pollen tubes.
• Supplemental Figure 6 - DAG co-localizes with FM4-64 to BrefeldinA (BFA) induced compartments.
• Supplemental Figure 7 - First, middle and last frames of supplemental movie 1
• Supplemental Figure 8 - First, middle and last frames of supplemental movie 2
• Supplemental Figure 9 - First, middle and last frames of supplemental movie 3
• Supplemental Movie Legends
• Supplemental Movie 1 - Dynamic imaging of NtPLC3 localization.
• Supplemental Movie 2 - Dynamic imaging of PI 4,5-P₂ localization.
• Supplemental Movie 3 - Dynamic imaging of DAG localization.