Supplemental Data

Files in this Data Supplement:

• Supplemental Table 3 - Distribution of observed and expected positive clones if multiple intragenic mutations function independently.
• Supplemental Table 4 - Distribution of positive clones with different numbers of non-synonymous mutations from the heavily mutagenized 3rd and 4th libraries.
• Supplemental Table 5 - Comparison of AGPase small and large subunits from unicellular green algae.
• Supplemental Figure 1 - Alignment of maize endosperm large (SH2) and small Subunit (BT2).
• Supplemental Figure 2 - Placement of point mutations created by error-prone PCR in Sh2 and Bt2 clones.
• Supplemental Figure 3 - Differential rates of evolution between Bt2 and Zea mays 2 after gene duplication.
• Supplemental Table 1 - Small and large AGPase subunit accession numbers.
• Supplemental Table 2 - Amino acid changes not tolerated in BT2 and SH2.