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Structural Evidence for the Evolution of Xyloglucanase Activity from Xyloglucan
Endo
-Transglycosylases: Biological Implications for Cell Wall Metabolism
Plant Cell Baumann et al. 19: 1947
Supplemental Data
Files in this Data Supplement:
Supplemental Figure 1
-
pH-Rate profile of Tm-NXG1.
Supplemental Figure 2
-
Ratio of initial rates of transglycosylation to hydrolysis catalyzed by Tm-NXG1 as a function of XGO2.
Supplemental Figure 3
-
Ratio of initial rates of transglycosylation to hydrolysis catalyzed by Tm-NXG1-δYNIIG as a function of XGO2.
Supplemental Figure 4
-
Full-length protein sequence alignment of Tm-NXG1, Tm-NXG2, Bob-XET16A, and Ptt-XET16-34.
Supplemental Figure 5
-
Partial protein sequence alignment of historical Group III gene products and Ptt-XET16-34.
Supplemental Table 1
-
Heterologously expressed members of the
XTH
gene family.
Supplemental Table 2
-
Unfolding temperature of Tm-NXG1 and Tm-NXG2.
Supplemental Table 3
-
Hetero-
transglycosylation products obtained from xyloglucan and cello-oligosaccharides.
Supplemental Text
-
Kinetic derivations
This Article
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