Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 3 - Complementation of the clsy1-3 and clsy1-4 mutants with a P_CLSY1:3xHA-CLSY1 transgene
• Supplemental Figure 1 - Structure of the T-DNA insertion site in the JAP 3 line
• Supplemental Figure 2 - Detection of endogenous siRNAs and miRNAs in spreading of silencing mutants
• Supplemental Figure 4 - Bioinformatic comparison of CLSY1 with other SNF2 domain-containing proteins
• Supplemental Figure 5 - Immunolocalisation of RDR2 and NRPD1a
• Supplemental Figure 6 - Immunolocalisation of DRD1 in a clsy1 mutant background
• Supplemental Figure 7 - Immunolocalisation of NRPD1b in a clsy1 mutant background
• Supplemental Table 1 - Isolation of new NRPD1a, RDR2 and DCL3 alleles
• Supplemental Table 2 - 5’ RACE of CLSY1
• Supplemental Table 3 - uORFs in the 5’ UTR of CLSY1
• Supplemental Table 4 - Immunolocalisation of P_CLSY1:3xHA-CLSY1 transgenic line and the 24nt pathway proteins in the clsy1 mutant background
• Supplemental Table 5 - Primer sequences