Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Complex N-glycan processing in Arabidopsis.
• Supplemental Table 2 - Primer sequences cited in Methods.
• Supplemental Figure 2 - Proteins from different organs of wildtype and cgl1 plants were analyzed by Western blot using JIM84.
• Supplemental Figure 3 - Western blot of transgenic lines expressing GALT candidate proteins.
• Supplemental Figure 4 - Amino acid sequence alignment and sequence identity between GALT1 and mammalian B3GALTs.
• Supplemental Figure 5 - N-glycan nomenclature, acronyms and symbols.
• Supplemental Figure 6 - Mn²⁺ dependency of GALT1.
• Supplemental Figure 7 - LC-MS data of recombinantly produced and purified GALT1 protein.
• Supplemental Figure 8 - MALDI-TOF MS of N-glycans isolated from glycoproteins of wildtype, galt1-1, 35S:GALT1 and galt1_RNAi stems.
• Supplemental Table 1 - 20 Arabidopsis sequences from CAZy family GT31 contain a galactosyltransferase domain (pfam 0176).