Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Immunogold labeling of wild type high-pressure frozen/ freeze substituted endosperm cells (22 DAP) using preimmune serum as a negative control for the immunolocalization of FL1. Preimmune serum was purified in the same way as FL1 immune antiserum as described in Methods. Scale bar = 500 nm
• Supplemental Table 1 - DUF593-containing proteins used for construction of phylogenetic tree.
• Supplemental Table 2 - +2 selective adaptor primers for SAIFF cloning of Fl1.
• Supplemental Table 3 - Primers for zein/pGADT7 constructs.