First published online January 29, 2002; 10.1105/tpc.010322
The Plant Cell, Vol. 14, 119-131,
January 2002, Copyright © 2002,
American Society of Plant Biologists
A Mutation in the Arabidopsis KT2/KUP2 Potassium Transporter Gene Affects Shoot Cell Expansion
Rangasamy P. Elumalai1,2,,a,
Punita Nagpal1,a and
Jason W. Reed1,3,a,b
a Department of Biology, University of North Carolina at Chapel Hill, Coker Hall, Chapel Hill, North Carolina 27599-3280
b Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
3 To whom correspondence should be addressed. E-mail jreed{at}email.unc.edu; fax 919-962-1625
Potassium ions (K+) are the most abundant cations in plants and are necessary for cell growth. Arabidopsis shy3-1 mutant plants have a short hypocotyl, small leaves, and a short flowering stem, and these defects result from decreased cell expansion. The semidominant shy3-1 mutation changes an amino acid in KT2/KUP2, a K+ transporter related to the Escherichia coli Kup protein. Second mutations in the KT2/KUP2/SHY3 gene, including presumed null mutations, suppress the shy3-1 phenotypes. Plants with these intragenic suppressor mutations appear similar to wild-type plants, suggesting that KT2/KUP2/SHY3 acts redundantly with other genes. Expression of the shy3-1 mutant version of KT2/KUP2/SHY3 in wild-type plants confers shy3-1–like phenotypes, indicating that shy3-1 probably either causes a gain of function or creates an interfering protein. The shy3-1 mutation does not eliminate the ability of the KT2/KUP2 cDNA to rescue the growth of a potassium transport-deficient E. coli mutant. A PSHY3::GUS fusion is expressed in growing portions of the plant. These results suggest that KT2/KUP2/SHY3 mediates K+-dependent cell expansion in growing tissues.
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