Plant Cell email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online November 20, 2002; 10.1105/tpc.006791

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
14/12/3057    most recent
tpc.006791v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Plant Cell
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (58)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by del Pozo, J. C.
Right arrow Articles by Gutierrez, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by del Pozo, J. C.
Right arrow Articles by Gutierrez, C.
Agricola
Right arrow Articles by del Pozo, J. C.
Right arrow Articles by Gutierrez, C.
The Plant Cell, Vol. 14, 3057-3071, December 2002, Copyright © 2002,
American Society of Plant Biologists

Arabidopsis E2Fc Functions in Cell Division and Is Degraded by the Ubiquitin-SCFAtSKP2 Pathway in Response to Light

Juan Carlos del Pozo, Maria Beatrice Boniotti and Crisanto Gutierrez1

Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco 28049, Madrid, Spain

1 To whom correspondence should be addressed. E-mail cgutierrez{at}cbm.uam.es; fax 34-9139-74799

Selective ubiquitin-mediated proteolysis through the cell cycle controls the availability, and therefore the activity, of several cell proliferation proteins. E2F transcription factors play distinct roles in both proliferating and differentiated cells by regulating gene expression. Here, we report that Arabidopsis AtE2Fc is regulated by a balance between gene expression and ubiquitin-proteasome proteolysis. AtE2Fc degradation implicates the function of the E3 ubiquitin-ligase Skp1, Cullin, F-box (SCFAtSKP2) complex and seems to be dependent on cyclin-dependent kinase phosphorylation. In addition, we found that AtE2Fc degradation is triggered by light stimulation of dark-grown seedlings. Interestingly, the auxin response mutant axr1-12, in which RUB1 modification of the SCF component CUL1 is impaired, shows increased AtE2Fc protein levels, suggesting a dysfunction in the control of AtE2Fc stability. Likewise, overexpression of a stable form of the AtE2Fc protein negatively affects cell division and increases cell size. These effects are mediated, at least in part, by downregulating the cell cycle gene AtCDC6. The negative role of AtE2Fc in gene expression is further supported by the fact that AtE2Fc interacts with plant retinoblastoma-related protein, suggesting that AtE2Fc might form part of a repressor complex. We propose that AtE2Fc might play a role in cell division and during the transition from skotomorphogenesis to photomorphogenesis.


Related articles in Plant Cell:

Ins and Outs of E2Fs
Nancy A. Eckardt
Plant Cell 2002 14: 2977-2980. [Full Text]  



This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. C. Day, R. P. Herridge, B. A. Ambrose, and R. C. Macknight
Transcriptome Analysis of Proliferating Arabidopsis Endosperm Reveals Biological Implications for the Control of Syncytial Division, Cytokinin Signaling, and Gene Expression Regulation
Plant Physiology, December 1, 2008; 148(4): 1964 - 1984.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Lopez-Juez, E. Dillon, Z. Magyar, S. Khan, S. Hazeldine, S. M. de Jager, J. A.H. Murray, G. T.S. Beemster, L. Bogre, and H. Shanahan
Distinct Light-Initiated Gene Expression and Cell Cycle Programs in the Shoot Apex and Cotyledons of Arabidopsis
PLANT CELL, April 1, 2008; 20(4): 947 - 968.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
W.D. Teale, F.A. Ditengou, A.D. Dovzhenko, X. Li, A.M. Molendijk, B. Ruperti, I. Paponov, and K. Palme
Auxin as a Model for the Integration of Hormonal Signal Processing and Transduction
Mol Plant, March 1, 2008; 1(2): 229 - 237.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. A. van den Burg, D. I. Tsitsigiannis, O. Rowland, J. Lo, G. Rallapalli, D. MacLean, F. L.W. Takken, and J. D.G. Jones
The F-Box Protein ACRE189/ACIF1 Regulates Cell Death and Defense Responses Activated during Pathogen Recognition in Tobacco and Tomato
PLANT CELL, March 1, 2008; 20(3): 697 - 719.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Ramirez-Parra and C. Gutierrez
E2F Regulates FASCIATA1, a Chromatin Assembly Gene Whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status
Plant Physiology, May 1, 2007; 144(1): 105 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Moon, Y. Zhao, X. Dai, W. Zhang, W. M. Gray, E. Huq, and M. Estelle
A New CULLIN 1 Mutant Has Altered Responses to Hormones and Light in Arabidopsis
Plant Physiology, February 1, 2007; 143(2): 684 - 696.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Adachi, H. Uchimiya, and M. Umeda
Expression of B2-Type Cyclin-Dependent Kinase is Controlled by Protein Degradation in Arabidopsis thaliana
Plant Cell Physiol., December 1, 2006; 47(12): 1683 - 1686.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Shen and L. Hanley-Bowdoin
Geminivirus Infection Up-Regulates the Expression of Two Arabidopsis Protein Kinases Related to Yeast SNF1- and Mammalian AMPK-Activating Kinases
Plant Physiology, December 1, 2006; 142(4): 1642 - 1655.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. C. del Pozo, S. Diaz-Trivino, N. Cisneros, and C. Gutierrez
The Balance between Cell Division and Endoreplication Depends on E2FC-DPB, Transcription Factors Regulated by the Ubiquitin-SCFSKP2A Pathway in Arabidopsis
PLANT CELL, September 1, 2006; 18(9): 2224 - 2235.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Sozzani, C. Maggio, S. Varotto, S. Canova, C. Bergounioux, D. Albani, and R. Cella
Interplay between Arabidopsis Activating Factors E2Fb and E2Fa in Cell Cycle Progression and Development
Plant Physiology, April 1, 2006; 140(4): 1355 - 1366.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Voigt and N. Papalopulu
A dominant-negative form of the E3 ubiquitin ligase Cullin-1 disrupts the correct allocation of cell fate in the neural crest lineage
Development, February 1, 2006; 133(3): 559 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Desvoyes, E. Ramirez-Parra, Q. Xie, N.-H. Chua, and C. Gutierrez
Cell Type-Specific Role of the Retinoblastoma/E2F Pathway during Arabidopsis Leaf Development
Plant Physiology, January 1, 2006; 140(1): 67 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. Huanca-Mamani, M. Garcia-Aguilar, G. Leon-Martinez, U. Grossniklaus, and J.-P. Vielle-Calzada
CHR11, a chromatin-remodeling factor essential for nuclear proliferation during female gametogenesis in Arabidopsis thaliana
PNAS, November 22, 2005; 102(47): 17231 - 17236.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Diaz-Trivino, M. del Mar Castellano, M. de la Paz Sanchez, E. Ramirez-Parra, B. Desvoyes, and C. Gutierrez
The genes encoding Arabidopsis ORC subunits are E2F targets and the two ORC1 genes are differently expressed in proliferating and endoreplicating cells
Nucleic Acids Res., September 22, 2005; 33(17): 5404 - 5414.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Magyar, L. De Veylder, A. Atanassova, L. Bako, D. Inze, and L. Bogre
The Role of the Arabidopsis E2FB Transcription Factor in Regulating Auxin-Dependent Cell Division
PLANT CELL, September 1, 2005; 17(9): 2527 - 2541.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Vandepoele, K. Vlieghe, K. Florquin, L. Hennig, G. T.S. Beemster, W. Gruissem, Y. Van de Peer, D. Inze, and L. De Veylder
Genome-Wide Identification of Potential Plant E2F Target Genes
Plant Physiology, September 1, 2005; 139(1): 316 - 328.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
S. Robbens, B. Khadaroo, A. Camasses, E. Derelle, C. Ferraz, D. Inze, Y. Van de Peer, and H. Moreau
Genome-Wide Analysis of Core Cell Cycle Genes in the Unicellular Green Alga Ostreococcus tauri
Mol. Biol. Evol., March 1, 2005; 22(3): 589 - 597.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Ramirez-Parra, M. A. Lopez-Matas, C. Frundt, and C. Gutierrez
Role of an Atypical E2F Transcription Factor in the Control of Arabidopsis Cell Growth and Differentiation
PLANT CELL, September 1, 2004; 16(9): 2350 - 2363.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. d. M. Castellano, M. B. Boniotti, E. Caro, A. Schnittger, and C. Gutierrez
DNA Replication Licensing Affects Cell Proliferation or Endoreplication in a Cell Type-Specific Manner
PLANT CELL, September 1, 2004; 16(9): 2380 - 2393.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Arguello-Astorga, L. Lopez-Ochoa, L.-J. Kong, B. M. Orozco, S. B. Settlage, and L. Hanley-Bowdoin
A Novel Motif in Geminivirus Replication Proteins Interacts with the Plant Retinoblastoma-Related Protein
J. Virol., May 1, 2004; 78(9): 4817 - 4826.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Ni, D. Xie, L. Hobbie, B. Feng, D. Zhao, J. Akkara, and H. Ma
Regulation of Flower Development in Arabidopsis by SCF Complexes
Plant Physiology, April 1, 2004; 134(4): 1574 - 1585.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C.-P. Lai, C.-L. Lee, P.-H. Chen, S.-H. Wu, C.-C. Yang, and J.-F. Shaw
Molecular Analyses of the Arabidopsis TUBBY-Like Protein Gene Family
Plant Physiology, April 1, 2004; 134(4): 1586 - 1597.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. Vlieghe, M. Vuylsteke, K. Florquin, S. Rombauts, S. Maes, S. Ormenese, P. Van Hummelen, Y. Van de Peer, D. Inze, and L. De Veylder
Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation
J. Cell Sci., October 15, 2003; 116(20): 4249 - 4259.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Zhao, W. Ni, B. Feng, T. Han, M. G. Petrasek, and H. Ma
Members of the Arabidopsis-SKP1-like Gene Family Exhibit a Variety of Expression Patterns and May Play Diverse Roles in Arabidopsis
Plant Physiology, September 1, 2003; 133(1): 203 - 217.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Kosugi and Y. Ohashi
Constitutive E2F Expression in Tobacco Plants Exhibits Altered Cell Cycle Control and Morphological Change in a Cell Type-Specific Manner
Plant Physiology, August 1, 2003; 132(4): 2012 - 2022.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Ins and Outs of E2Fs
PLANT CELL, December 1, 2002; 14(12): 2977 - 2980.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2002 by the American Society of Plant Biologists