Plant Cell email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online January 17, 2003; 10.1105/tpc.008342

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
15/2/303    most recent
tpc.008342v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (53)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schnittger, A.
Right arrow Articles by Hülskamp, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schnittger, A.
Right arrow Articles by Hülskamp, M.
Agricola
Right arrow Articles by Schnittger, A.
Right arrow Articles by Hülskamp, M.
The Plant Cell, Vol. 15, 303-315, February 2003, Copyright © 2003,
American Society of Plant Biologists

Misexpression of the Cyclin-Dependent Kinase Inhibitor ICK1/KRP1 in Single-Celled Arabidopsis Trichomes Reduces Endoreduplication and Cell Size and Induces Cell Death

Arp Schnittger1,2,a,b, Christina Weinlb,c, Daniel Bouyerb, Ulrike Schöbinger1,a,b and Martin Hülskamp2,b

a Zentrum für Molekularbiologie der Pflanzen, Universität Tübingen, Auf der Morgenstelle 3, 72076 Tübingen, Germany
b Botanisches Institut 3, Universität Köln, Gyrhofstrasse 15, 50931 Köln, Germany
c Unigruppe, Max-Planck-Institut für Züchtungsforschung, Carl von Linné Weg 10, 50829 Köln, Germany

2 To whom correspondence should be addressed. E-mail schnitt{at}mpiz-koeln.mpg.de; fax 49-0-221-5062-113; or e-mail martin.huelskamp{at}uni-koeln.mpg.de; fax 49-0-221-470-5062

A positive correlation between cell size and DNA content has been recognized in many plant cell types. Conversely, misexpression of a dominant-negative cyclin-dependent kinase (CDK) or CDK inhibitor proteins (ICK/KRPs) in Arabidopsis and tobacco leaves has revealed that cell growth can be uncoupled from cell cycle progression and DNA content. However, cell growth also appears to be controlled in a non-cell-autonomous manner by organ size, making it difficult in a ubiquitous expression assay to judge the cell-autonomous function of putative cell growth regulators. Here, we investigated the function of the CDK inhibitor ICK1/KRP1 on cell growth and differentiation independent of any compensatory influence of an organ context using Arabidopsis trichomes as a model system. By analyzing cell size with respect to DNA content, we dissected cell growth in a DNA-dependent and a DNA-independent process. We further found that ICK1/KRP1 misexpression interfered with differentiation and induced cell death, linking cell cycle progression, differentiation, and cell death in plants. The function of ICK1/KRP1 in planta was found to be dependent on a C-terminal domain and regulated negatively by an N-terminal domain. Finally, we identified CDKA;1 and a D-type cyclin as possible targets of ICK1/KRP1 expression in vivo.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. J. Jakoby, D. Falkenhan, M. T. Mader, G. Brininstool, E. Wischnitzki, N. Platz, A. Hudson, M. Hulskamp, J. Larkin, and A. Schnittger
Transcriptional Profiling of Mature Arabidopsis Trichomes Reveals That NOECK Encodes the MIXTA-Like Transcriptional Regulator MYB106
Plant Physiology, November 1, 2008; 148(3): 1583 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Tominaga, M. Iwata, R. Sano, K. Inoue, K. Okada, and T. Wada
Arabidopsis CAPRICE-LIKE MYB 3 (CPL3) controls endoreduplication and flowering development in addition to trichome and root hair formation
Development, April 1, 2008; 135(7): 1335 - 1345.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Guimil and C. Dunand
Cell growth and differentiation in Arabidopsis epidermal cells
J. Exp. Bot., November 1, 2007; 58(14): 3829 - 3840.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Ferjani, G. Horiguchi, S. Yano, and H. Tsukaya
Analysis of Leaf Development in fugu Mutants of Arabidopsis Reveals Three Compensation Modes That Modulate Cell Expansion in Determinate Organs
Plant Physiology, June 1, 2007; 144(2): 988 - 999.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Dissmeyer, M. K. Nowack, S. Pusch, H. Stals, D. Inze, P. E. Grini, and A. Schnittger
T-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate Residue
PLANT CELL, March 1, 2007; 19(3): 972 - 985.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. M. Barroco, A. Peres, A.-M. Droual, L. De Veylder, L. S. L. Nguyen, J. De Wolf, V. Mironov, R. Peerbolte, G. T.S. Beemster, D. Inze, et al.
The Cyclin-Dependent Kinase Inhibitor Orysa;KRP1 Plays an Important Role in Seed Development of Rice
Plant Physiology, November 1, 2006; 142(3): 1053 - 1064.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Serralbo, J. M. Perez-Perez, R. Heidstra, and B. Scheres
Non-cell-autonomous rescue of anaphase-promoting complex function revealed by mosaic analysis of HOBBIT, an Arabidopsis CDC27 homolog
PNAS, August 29, 2006; 103(35): 13250 - 13255.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. Jakoby, C. Weinl, S. Pusch, S. J.H. Kuijt, T. Merkle, N. Dissmeyer, and A. Schnittger
Analysis of the Subcellular Localization, Function, and Proteolytic Control of the Arabidopsis Cyclin-Dependent Kinase Inhibitor ICK1/KRP1
Plant Physiology, August 1, 2006; 141(4): 1293 - 1305.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Bisbis, F. Delmas, J. Joubes, A. Sicard, M. Hernould, D. Inze, A. Mouras, and C. Chevalier
Cyclin-dependent Kinase (CDK) Inhibitors Regulate the CDK-Cyclin Complex Activities in Endoreduplicating Cells of Developing Tomato Fruit
J. Biol. Chem., March 17, 2006; 281(11): 7374 - 7383.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Correa-Aragunde, M. Graziano, C. Chevalier, and L. Lamattina
Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato
J. Exp. Bot., February 1, 2006; 57(3): 581 - 588.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Kurdyukov, A. Faust, C. Nawrath, S. Bar, D. Voisin, N. Efremova, R. Franke, L. Schreiber, H. Saedler, J.-P. Metraux, et al.
The Epidermis-Specific Extracellular BODYGUARD Controls Cuticle Development and Morphogenesis in Arabidopsis
PLANT CELL, February 1, 2006; 18(2): 321 - 339.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. K. Imai, Y. Ohashi, T. Tsuge, T. Yoshizumi, M. Matsui, A. Oka, and T. Aoyama
The A-Type Cyclin CYCA2;3 Is a Key Regulator of Ploidy Levels in Arabidopsis Endoreduplication
PLANT CELL, February 1, 2006; 18(2): 382 - 396.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Desvoyes, E. Ramirez-Parra, Q. Xie, N.-H. Chua, and C. Gutierrez
Cell Type-Specific Role of the Retinoblastoma/E2F Pathway during Arabidopsis Leaf Development
Plant Physiology, January 1, 2006; 140(1): 67 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Sugimoto-Shirasu, G. R. Roberts, N. J. Stacey, M. C. McCann, A. Maxwell, and K. Roberts
RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth
PNAS, December 20, 2005; 102(51): 18736 - 18741.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Verkest, C. Weinl, D. Inze, L. De Veylder, and A. Schnittger
Switching the Cell Cycle. Kip-Related Proteins in Plant Cell Cycle Control
Plant Physiology, November 1, 2005; 139(3): 1099 - 1106.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. M. Coelho, R. A. Dante, P. A. Sabelli, Y. Sun, B. P. Dilkes, W. J. Gordon-Kamm, and B. A. Larkins
Cyclin-Dependent Kinase Inhibitors in Maize Endosperm and Their Potential Role in Endoreduplication
Plant Physiology, August 1, 2005; 138(4): 2323 - 2336.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Raynaud, C. Perennes, C. Reuzeau, O. Catrice, S. Brown, and C. Bergounioux
Cell and plastid division are coordinated through the prereplication factor AtCDT1
PNAS, June 7, 2005; 102(23): 8216 - 8221.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Weinl, S. Marquardt, S. J.H. Kuijt, M. K. Nowack, M. J. Jakoby, M. Hulskamp, and A. Schnittger
Novel Functions of Plant Cyclin-Dependent Kinase Inhibitors, ICK1/KRP1, Can Act Non-Cell-Autonomously and Inhibit Entry into Mitosis
PLANT CELL, June 1, 2005; 17(6): 1704 - 1722.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Verkest, C.-L. d. O. Manes, S. Vercruysse, S. Maes, E. Van Der Schueren, T. Beeckman, P. Genschik, M. Kuiper, D. Inze, and L. De Veylder
The Cyclin-Dependent Kinase Inhibitor KRP2 Controls the Onset of the Endoreduplication Cycle during Arabidopsis Leaf Development through Inhibition of Mitotic CDKA;1 Kinase Complexes
PLANT CELL, June 1, 2005; 17(6): 1723 - 1736.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Lu, Y.-R. J. Lee, R. Pan, J. N. Maloof, and B. Liu
An Internal Motor Kinesin Is Associated with the Golgi Apparatus and Plays a Role in Trichome Morphogenesis in Arabidopsis
Mol. Biol. Cell, February 1, 2005; 16(2): 811 - 823.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kozuka, G. Horiguchi, G.-T. Kim, M. Ohgishi, T. Sakai, and H. Tsukaya
The Different Growth Responses of the Arabidopsis thaliana Leaf Blade and the Petiole during Shade Avoidance are Regulated by Photoreceptors and Sugar
Plant Cell Physiol., January 15, 2005; 46(1): 213 - 223.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Yu, A. Steinmetz, D. Meyer, S. Brown, and W.-H. Shen
The Tobacco A-Type Cyclin, Nicta;CYCA3;2, at the Nexus of Cell Division and Differentiation
PLANT CELL, December 1, 2003; 15(12): 2763 - 2777.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists