Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online January 23, 2003; 10.1105/tpc.008425

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
15/2/523    most recent
tpc.008425v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burget, E. G.
Right arrow Articles by Reiter, W.-D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burget, E. G.
Right arrow Articles by Reiter, W.-D.
Agricola
Right arrow Articles by Burget, E. G.
Right arrow Articles by Reiter, W.-D.
The Plant Cell, Vol. 15, 523-531, February 2003, Copyright © 2003,
American Society of Plant Biologists

The Biosynthesis of L-Arabinose in Plants

Molecular Cloning and Characterization of a Golgi-Localized UDP-D-Xylose 4-Epimerase Encoded by the MUR4 Gene of Arabidopsis

Emilie G. Burget, Rajeev Verma, Michael Mølhøj and Wolf-Dieter Reiter1

Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269

1 To whom correspondence should be addressed. E-mail wdreiter{at}uconnvm.uconn.edu; fax 860-486-4331

The mur4 mutant of Arabidopsis shows a 50% reduction in the monosaccharide L-Ara in leaf-derived cell wall material because of a partial defect in the 4-epimerization of UDP-D-Xyl to UDP-L-Ara. To determine the genetic lesion underlying the mur4 phenotype, the MUR4 gene was cloned by a map-based procedure and found to encode a type-II membrane protein with sequence similarity to UDP-D-Glc 4-epimerases. Enzyme assays of MUR4 protein expressed in the methylotropic yeast Pichia pastoris indicate that it catalyzes the 4-epimerization of UDP-D-Xyl to UDP-L-Ara, the nucleotide sugar used by glycosyltransferases in the arabinosylation of cell wall polysaccharides and wall-resident proteoglycans. Expression of MUR4–green fluorescent protein constructs in Arabidopsis revealed localization patterns consistent with targeting to the Golgi, suggesting that the MUR4 protein colocalizes with glycosyltransferases in the biosynthesis of arabinosylated cell wall components. The Arabidopsis genome encodes three putative proteins with >76% sequence identity to MUR4, which may explain why mur4 plants are not entirely deficient in the de novo synthesis of UDP-L-Ara.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. A. Chavez Montes, P. Ranocha, Y. Martinez, Z. Minic, L. Jouanin, M. Marquis, L. Saulnier, L. M. Fulton, C. S. Cobbett, F. Bitton, et al.
Cell Wall Modifications in Arabidopsis Plants with Altered {alpha}-L-Arabinofuranosidase Activity
Plant Physiology, May 1, 2008; 147(1): 63 - 77.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Zeng, M. Chatterjee, and A. Faik
UDP-Xylose-Stimulated Glucuronyltransferase Activity in Wheat Microsomal Membranes: Characterization and Role in Glucurono(arabino)xylan Biosynthesis
Plant Physiology, May 1, 2008; 147(1): 78 - 91.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-C. Leple, R. Dauwe, K. Morreel, V. Storme, C. Lapierre, B. Pollet, A. Naumann, K.-Y. Kang, H. Kim, K. Ruel, et al.
Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure
PLANT CELL, November 1, 2007; 19(11): 3669 - 3691.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Klinghammer and R. Tenhaken
Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls
J. Exp. Bot., October 1, 2007; 58(13): 3609 - 3621.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Li, C. Smith, F. Corke, L. Zheng, Z. Merali, P. Ryden, P. Derbyshire, K. Waldron, and M. W. Bevan
Signaling from an Altered Cell Wall to the Nucleus Mediates Sugar-Responsive Growth and Development in Arabidopsis thaliana
PLANT CELL, August 1, 2007; 19(8): 2500 - 2515.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G.-K. Zhou, R. Zhong, D. S. Himmelsbach, B. T. McPhail, and Z.-H. Ye
Molecular Characterization of PoGT8D and PoGT43B, Two Secondary Wall-Associated Glycosyltransferases in Poplar
Plant Cell Physiol., May 1, 2007; 48(5): 689 - 699.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Rosti, C. J. Barton, S. Albrecht, P. Dupree, M. Pauly, K. Findlay, K. Roberts, and G. J. Seifert
UDP-Glucose 4-Epimerase Isoforms UGE2 and UGE4 Cooperate in Providing UDP-Galactose for Cell Wall Biosynthesis and Growth of Arabidopsis thaliana
PLANT CELL, May 1, 2007; 19(5): 1565 - 1579.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Oka, T. Nemoto, and Y. Jigami
Functional Analysis of Arabidopsis thaliana RHM2/MUM4, a Multidomain Protein Involved in UDP-D-glucose to UDP-L-rhamnose Conversion
J. Biol. Chem., February 23, 2007; 282(8): 5389 - 5403.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. J. Pena, R. Zhong, G.-K. Zhou, E. A. Richardson, M. A. O'Neill, A. G. Darvill, W. S. York, and Z.-H. Ye
Arabidopsis irregular xylem8 and irregular xylem9: Implications for the Complexity of Glucuronoxylan Biosynthesis
PLANT CELL, February 1, 2007; 19(2): 549 - 563.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Egelund, B. L. Petersen, M. S. Motawia, I. Damager, A. Faik, C. E. Olsen, T. Ishii, H. Clausen, P. Ulvskov, and N. Geshi
Arabidopsis thaliana RGXT1 and RGXT2 Encode Golgi-Localized (1,3)-{alpha}-D-Xylosyltransferases Involved in the Synthesis of Pectic Rhamnogalacturonan-II
PLANT CELL, October 1, 2006; 18(10): 2593 - 2607.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G.-K. Zhou, R. Zhong, E. A. Richardson, W. H. Morrison III, C. J. Nairn, A. Wood-Jones, and Z.-H. Ye
The Poplar Glycosyltransferase GT47C is Functionally Conserved with Arabidopsis Fragile Fiber8
Plant Cell Physiol., September 1, 2006; 47(9): 1229 - 1240.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Mouille, H. Witucka-Wall, M.-P. Bruyant, O. Loudet, S. Pelletier, C. Rihouey, O. Lerouxel, P. Lerouge, H. Hofte, and M. Pauly
Quantitative Trait Loci Analysis of Primary Cell Wall Composition in Arabidopsis
Plant Physiology, July 1, 2006; 141(3): 1035 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. D. Sterling, M. A. Atmodjo, S. E. Inwood, V. S. Kumar Kolli, H. F. Quigley, M. G. Hahn, and D. Mohnen
From the Cover: Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase
PNAS, March 28, 2006; 103(13): 5236 - 5241.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Zhong, M. J. Pena, G.-K. Zhou, C. J. Nairn, A. Wood-Jones, E. A. Richardson, W. H. Morrison III, A. G. Darvill, W. S. York, and Z.-H. Ye
Arabidopsis Fragile Fiber8, Which Encodes a Putative Glucuronyltransferase, Is Essential for Normal Secondary Wall Synthesis
PLANT CELL, December 1, 2005; 17(12): 3390 - 3408.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Tzafrir, R. Pena-Muralla, A. Dickerman, M. Berg, R. Rogers, S. Hutchens, T. C. Sweeney, J. McElver, G. Aux, D. Patton, et al.
Identification of Genes Required for Embryo Development in Arabidopsis
Plant Physiology, July 1, 2004; 135(3): 1206 - 1220.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Molhoj, R. Verma, and W.-D. Reiter
The Biosynthesis of D-Galacturonate in Plants. Functional Cloning and Characterization of a Membrane-Anchored UDP-D-Glucuronate 4-Epimerase from Arabidopsis
Plant Physiology, July 1, 2004; 135(3): 1221 - 1230.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Li, I. Cordero, J. Caplan, M. Molhoj, and W.-D. Reiter
Molecular Analysis of 10 Coding Regions from Arabidopsis That Are Homologous to the MUR3 Xyloglucan Galactosyltransferase
Plant Physiology, March 1, 2004; 134(3): 940 - 950.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Madson, C. Dunand, X. Li, R. Verma, G. F. Vanzin, J. Caplan, D. A. Shoue, N. C. Carpita, and W.-D. Reiter
The MUR3 Gene of Arabidopsis Encodes a Xyloglucan Galactosyltransferase That Is Evolutionarily Related to Animal Exostosins
PLANT CELL, July 1, 2003; 15(7): 1662 - 1670.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists