Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online February 24, 2003; 10.1105/tpc.008961

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
15/3/597    most recent
tpc.008961v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (65)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dhonukshe, P.
Right arrow Articles by Gadella, T. W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dhonukshe, P.
Right arrow Articles by Gadella, T. W. J., Jr.
Agricola
Right arrow Articles by Dhonukshe, P.
Right arrow Articles by Gadella, T. W. J.
The Plant Cell, Vol. 15, 597-611, March 2003, Copyright © 2003,
American Society of Plant Biologists

Alteration of Microtubule Dynamic Instability during Preprophase Band Formation Revealed by Yellow Fluorescent Protein–CLIP170 Microtubule Plus-End Labeling

Pankaj Dhonukshe and Theodorus W. J. Gadella, Jr.1

Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, PO Box 94062, NL-1090 GB Amsterdam, The Netherlands

1 To whom correspondence should be addressed. E-mail gadella{at}science.uva.nl; fax 31-20-5256271

At the onset of mitosis, plant cells form a microtubular preprophase band that defines the plane of cell division, but the mechanism of its formation remains a mystery. Here, we describe the use of mammalian yellow fluorescent protein–tagged CLIP170 to visualize the dynamic plus ends of plant microtubules in transfected cowpea protoplasts and in stably transformed and dividing tobacco Bright Yellow 2 cells. Using plus-end labeling, we observed dynamic instability in different microtubular conformations in live plant cells. The interphase plant microtubules grow at 5 µm/min, shrink at 20 µm/min, and display catastrophe and rescue frequencies of 0.02 and 0.08 events/s, respectively, exhibiting faster turnover than their mammalian counterparts. Strikingly, during preprophase band formation, the growth rate and catastrophe frequency of plant microtubules double, whereas the shrinkage rate and rescue frequency remain unchanged, making microtubules shorter and more dynamic. Using these novel insights and four-dimensional time-lapse imaging data, we propose a model that can explain the mechanism by which changes in microtubule dynamic instability drive the dramatic rearrangements of microtubules during preprophase band and spindle formation in plant cells.




This article has been cited by other articles:


Home page
 Mol PlantHome page
J. C. Ambrose and R. Cyr
Mitotic Spindle Organization by the Preprophase Band
Mol Plant, November 1, 2008; 1(6): 950 - 960.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. C. Ambrose, T. Shoji, A. M. Kotzer, J. A. Pighin, and G. O. Wasteneys
The Arabidopsis CLASP Gene Encodes a Microtubule-Associated Protein Involved in Cell Expansion and Division
PLANT CELL, September 1, 2007; 19(9): 2763 - 2775.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. C. Lagomarsino, C. Tanase, J. W. Vos, A. M. C. Emons, B. M. Mulder, and M. Dogterom
Microtubule Organization in Three-Dimensional Confined Geometries: Evaluating the Role of Elasticity Through a Combined In Vitro and Modeling Approach
Biophys. J., February 1, 2007; 92(3): 1046 - 1057.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
P. Dhonukshe, B. O.R. Bargmann, and T. W.J. Gadella Jr
Arabidopsis Tubulin Folding Cofactor B Interacts with {alpha}-Tubulin In Vivo
Plant Cell Physiol., October 1, 2006; 47(10): 1406 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Dhonukshe, N. Vischer, and T. W. J. Gadella Jr
Contribution of microtubule growth polarity and flux to spindle assembly and functioning in plant cells.
J. Cell Sci., August 1, 2006; 119(Pt 15): 3193 - 3205.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Hirase, T. Hamada, T. J. Itoh, T. Shimmen, and S. Sonobe
n-Butanol induces depolymerization of microtubules in vivo and in vitro
Plant Cell Physiol., July 1, 2006; 47(7): 1004 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Dixit, E. Chang, and R. Cyr
Establishment of Polarity during Organization of the Acentrosomal Plant Cortical Microtubule Array
Mol. Biol. Cell, March 1, 2006; 17(3): 1298 - 1305.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Hause, S. Lischewski, L. A. Wessjohann, and B. Hause
Epothilone D affects cell cycle and microtubular pattern in plant cells
J. Exp. Bot., August 1, 2005; 56(418): 2131 - 2137.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H.-Y. Chang, A. P. Smertenko, H. Igarashi, D. P. Dixon, and P. J. Hussey
Dynamic interaction of NtMAP65-1a with microtubules in vivo
J. Cell Sci., July 15, 2005; 118(14): 3195 - 3201.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. N. Vassileva, H. Kouchi, and R. W. Ridge
Microtubule Dynamics in Living Root Hairs: Transient Slowing by Lipochitin Oligosaccharide Nodulation Signals
PLANT CELL, June 1, 2005; 17(6): 1777 - 1787.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Chan, G. Calder, S. Fox, and C. Lloyd
Localization of the Microtubule End Binding Protein EB1 Reveals Alternative Pathways of Spindle Development in Arabidopsis Suspension Cells
PLANT CELL, June 1, 2005; 17(6): 1737 - 1748.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. O. Wasteneys and Z. Yang
New Views on the Plant Cytoskeleton
Plant Physiology, December 1, 2004; 136(4): 3884 - 3891.
[Full Text] [PDF]

Home page
 Plant CellHome page
R. Dixit and R. Cyr
Encounters between Dynamic Cortical Microtubules Promote Ordering of the Cortical Array through Angle-Dependent Modifications of Microtubule Behavior
PLANT CELL, December 1, 2004; 16(12): 3274 - 3284.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Shoji, N. N. Narita, K. Hayashi, J. Asada, T. Hamada, S. Sonobe, K. Nakajima, and T. Hashimoto
Plant-Specific Microtubule-Associated Protein SPIRAL2 Is Required for Anisotropic Growth in Arabidopsis
Plant Physiology, December 1, 2004; 136(4): 3933 - 3944.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Van Damme, K. Van Poucke, E. Boutant, C. Ritzenthaler, D. Inze, and D. Geelen
In Vivo Dynamics and Differential Microtubule-Binding Activities of MAP65 Proteins
Plant Physiology, December 1, 2004; 136(4): 3956 - 3967.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Van Bruaene, G. Joss, and P. Van Oostveldt
Reorganization and in Vivo Dynamics of Microtubules during Arabidopsis Root Hair Development
Plant Physiology, December 1, 2004; 136(4): 3905 - 3919.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Dixit and R. Cyr
The Cortical Microtubule Array: From Dynamics to Organization
PLANT CELL, October 1, 2004; 16(10): 2546 - 2552.
[Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Nakamura, K. Naoi, T. Shoji, and T. Hashimoto
Low Concentrations of Propyzamide and Oryzalin Alter Microtubule Dynamics in Arabidopsis Epidermal Cells
Plant Cell Physiol., September 15, 2004; 45(9): 1330 - 1334.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. C. Sedbrook, D. W. Ehrhardt, S. E. Fisher, W.-R. Scheible, and C. R. Somerville
The Arabidopsis SKU6/SPIRAL1 Gene Encodes a Plus End-Localized Microtubule-Interacting Protein Involved in Directional Cell Expansion
PLANT CELL, June 1, 2004; 16(6): 1506 - 1520.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Dhonukshe, A. M. Laxalt, J. Goedhart, T. W. J. Gadella, and T. Munnik
Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells
PLANT CELL, November 1, 2003; 15(11): 2666 - 2679.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists