Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online February 21, 2003; 10.1105/tpc.008433

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
15/3/612    most recent
tpc.008433v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (73)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Willemsen, V.
Right arrow Articles by Scheres, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Willemsen, V.
Right arrow Articles by Scheres, B.
Agricola
Right arrow Articles by Willemsen, V.
Right arrow Articles by Scheres, B.
The Plant Cell, Vol. 15, 612-625, March 2003, Copyright © 2003,
American Society of Plant Biologists

Cell Polarity and PIN Protein Positioning in Arabidopsis Require STEROL METHYLTRANSFERASE1 Function

Viola Willemsena, Jirí Friml1,b, Markus Grebea, Albert van den Toorna, Klaus Palmec and Ben Scheres2,a

a Developmental Genetics, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
b Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft, Carl-von-Linné-Weg 10, 50829 Köln, Germany
c Institut für Biologie II, Zell-biologie, Universität Freiburg, 79104 Freiburg, Germany

2 To whom correspondence should be addressed. E-mail b.scheres{at}bio.uu.nl; fax 31-30-2513655

Plants have many polarized cell types, but relatively little is known about the mechanisms that establish polarity. The orc mutant was identified originally by defects in root patterning, and positional cloning revealed that the affected gene encodes STEROL METHYLTRANSFERASE1, which is required for the appropriate synthesis and composition of major membrane sterols. smt1orc mutants displayed several conspicuous cell polarity defects. Columella root cap cells revealed perturbed polar positioning of different organelles, and in the smt1orc root epidermis, polar initiation of root hairs was more randomized. Polar auxin transport and expression of the auxin reporter DR5-{beta}-glucuronidase were aberrant in smt1orc. Patterning defects in smt1orc resembled those observed in mutants of the PIN gene family of putative auxin efflux transporters. Consistently, the membrane localization of the PIN1 and PIN3 proteins was disturbed in smt1orc, whereas polar positioning of the influx carrier AUX1 appeared normal. Our results suggest that balanced sterol composition is a major requirement for cell polarity and auxin efflux in Arabidopsis.




This article has been cited by other articles:


Home page
 Mol PlantHome page
X. Gao, S. Nagawa, G. Wang, and Z. Yang
Cell Polarity Signaling: Focus on Polar Auxin Transport
Mol Plant, November 20, 2008; (2008) ssn069v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Titapiwatanakun and A. S. Murphy
Post-transcriptional regulation of auxin transport proteins: cellular trafficking, protein phosphorylation, protein maturation, ubiquitination, and membrane composition
J. Exp. Bot., September 29, 2008; (2008) ern240v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Feraru and J. Friml
PIN Polar Targeting
Plant Physiology, August 1, 2008; 147(4): 1553 - 1559.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. A. Konopka and S. Y. Bednarek
Comparison of the Dynamics and Functional Redundancy of the Arabidopsis Dynamin-Related Isoforms DRP1A and DRP1C during Plant Development
Plant Physiology, August 1, 2008; 147(4): 1590 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Muller, U. Mettbach, D. Menzel, and J. Samaj
Molecular Dissection of Endosomal Compartments in Plants
Plant Physiology, October 1, 2007; 145(2): 293 - 304.
[Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Kobayashi, M. Suzuki, J. Tang, N. Nagata, K. Ohyama, H. Seki, R. Kiuchi, Y. Kaneko, M. Nakazawa, M. Matsui, et al.
LOVASTATIN INSENSITIVE 1, a Novel Pentatricopeptide Repeat Protein, is a Potential Regulatory Factor of Isoprenoid Biosynthesis in Arabidopsis
Plant Cell Physiol., February 1, 2007; 48(2): 322 - 331.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Moreau, F. Brandizzi, S. Hanton, L. Chatre, S. Melser, C. Hawes, and B. Satiat-Jeunemaitre
The plant ER-Golgi interface: a highly structured and dynamic membrane complex
J. Exp. Bot., January 1, 2007; 58(1): 49 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Laloi, A.-M. Perret, L. Chatre, S. Melser, C. Cantrel, M.-N. Vaultier, A. Zachowski, K. Bathany, J.-M. Schmitter, M. Vallet, et al.
Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells
Plant Physiology, January 1, 2007; 143(1): 461 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Kleine-Vehn, P. Dhonukshe, R. Swarup, M. Bennett, and J. Friml
Subcellular Trafficking of the Arabidopsis Auxin Influx Carrier AUX1 Uses a Novel Pathway Distinct from PIN1
PLANT CELL, November 1, 2006; 18(11): 3171 - 3181.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Jin, S. Li, and A. Villegas Jr.
Down-Regulation of the 26S Proteasome Subunit RPN9 Inhibits Viral Systemic Transport and Alters Plant Vascular Development
Plant Physiology, October 1, 2006; 142(2): 651 - 661.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
G. Grossmann, M. Opekarova, L. Novakova, J. Stolz, and W. Tanner
Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae
Eukaryot. Cell, June 1, 2006; 5(6): 945 - 953.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. E. Sieburth, G. K. Muday, E. J. King, G. Benton, S. Kim, K. E. Metcalf, L. Meyers, E. Seamen, and J. M. Van Norman
SCARFACE Encodes an ARF-GAP That Is Required for Normal Auxin Efflux and Vein Patterning in Arabidopsis
PLANT CELL, June 1, 2006; 18(6): 1396 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. Boutte, M.-T. Crosnier, N. Carraro, J. Traas, and B. Satiat-Jeunemaitre
The plasma membrane recycling pathway and cell polarity in plants: studies on PIN proteins
J. Cell Sci., April 1, 2006; 119(7): 1255 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Morikawa, M. Mizutani, N. Aoki, B. Watanabe, H. Saga, S. Saito, A. Oikawa, H. Suzuki, N. Sakurai, D. Shibata, et al.
Cytochrome P450 CYP710A Encodes the Sterol C-22 Desaturase in Arabidopsis and Tomato
PLANT CELL, April 1, 2006; 18(4): 1008 - 1022.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Li, J. Xu, Z.-H. Xu, and H.-W. Xue
Brassinosteroids Stimulate Plant Tropisms through Modulation of Polar Auxin Transport in Brassica and Arabidopsis
PLANT CELL, October 1, 2005; 17(10): 2738 - 2753.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. O'Brien, S.-C. Chantha, A. Rahier, and D. P. Matton
Lipid Signaling in Plants. Cloning and Expression Analysis of the Obtusifoliol 14{alpha}-Demethylase from Solanum chacoense Bitt., a Pollination- and Fertilization-Induced Gene with Both Obtusifoliol and Lanosterol Demethylase Activity
Plant Physiology, October 1, 2005; 139(2): 734 - 749.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. I.A. Calderon-Villalobos, C. Kuhnle, E. M.N. Dohmann, H. Li, M. Bevan, and C. Schwechheimer
The Evolutionarily Conserved TOUGH Protein Is Required for Proper Development of Arabidopsis thaliana
PLANT CELL, September 1, 2005; 17(9): 2473 - 2485.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. B. Kim, H. Schaller, C.-H. Goh, M. Kwon, S. Choe, C. S. An, F. Durst, K. A. Feldmann, and R. Feyereisen
Arabidopsis cyp51 Mutant Shows Postembryonic Seedling Lethality Associated with Lack of Membrane Integrity
Plant Physiology, August 1, 2005; 138(4): 2033 - 2047.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. K. Clay and T. Nelson
Arabidopsis thickvein Mutation Affects Vein Thickness and Organ Vascularization, and Resides in a Provascular Cell-Specific Spermine Synthase Involved in Vein Definition and in Polar Auxin Transport
Plant Physiology, June 1, 2005; 138(2): 767 - 777.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Sawa, K. Koizumi, S. Naramoto, T. Demura, T. Ueda, A. Nakano, and H. Fukuda
DRP1A Is Responsible for Vascular Continuity Synergistically Working with VAN3 in Arabidopsis
Plant Physiology, June 1, 2005; 138(2): 819 - 826.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Zheng, O. Rowland, and L. Kunst
Disruptions of the Arabidopsis Enoyl-CoA Reductase Gene Reveal an Essential Role for Very-Long-Chain Fatty Acid Synthesis in Cell Expansion during Plant Morphogenesis
PLANT CELL, May 1, 2005; 17(5): 1467 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Koizumi, S. Naramoto, S. Sawa, N. Yahara, T. Ueda, A. Nakano, M. Sugiyama, and H. Fukuda
VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation
Development, April 1, 2005; 132(7): 1699 - 1711.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Bloch, M. Lavy, Y. Efrat, I. Efroni, K. Bracha-Drori, M. Abu-Abied, E. Sadot, and S. Yalovsky
Ectopic Expression of an Activated RAC in Arabidopsis Disrupts Membrane Cycling
Mol. Biol. Cell, April 1, 2005; 16(4): 1913 - 1927.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. H.H. Borner, D. J. Sherrier, T. Weimar, L. V. Michaelson, N. D. Hawkins, A. MacAskill, J. A. Napier, M. H. Beale, K. S. Lilley, and P. Dupree
Analysis of Detergent-Resistant Membranes in Arabidopsis. Evidence for Plasma Membrane Lipid Rafts
Plant Physiology, January 1, 2005; 137(1): 104 - 116.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Cano-Delgado, Y. Yin, C. Yu, D. Vafeados, S. Mora-Garcia, J.-C. Cheng, K. H. Nam, J. Li, and J. Chory
BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis
Development, November 1, 2004; 131(21): 5341 - 5351.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Swarup, J. Kargul, A. Marchant, D. Zadik, A. Rahman, R. Mills, A. Yemm, S. May, L. Williams, P. Millner, et al.
Structure-Function Analysis of the Presumptive Arabidopsis Auxin Permease AUX1
PLANT CELL, November 1, 2004; 16(11): 3069 - 3083.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. Scarpella, P. Francis, and T. Berleth
Stage-specific markers define early steps of procambium development in Arabidopsis leaves and correlate termination of vein formation with mesophyll differentiation
Development, July 15, 2004; 131(14): 3445 - 3455.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Samaj, F. Baluska, B. Voigt, M. Schlicht, D. Volkmann, and D. Menzel
Endocytosis, Actin Cytoskeleton, and Signaling
Plant Physiology, July 1, 2004; 135(3): 1150 - 1161.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Tantikanjana, M. D. Mikkelsen, M. Hussain, B. A. Halkier, and V. Sundaresan
Functional Analysis of the Tandem-Duplicated P450 Genes SPS/BUS/CYP79F1 and CYP79F2 in Glucosinolate Biosynthesis and Plant Development by Ds Transposition-Generated Double Mutants
Plant Physiology, June 1, 2004; 135(2): 840 - 848.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Laux, T. Wurschum, and H. Breuninger
Genetic Regulation of Embryonic Pattern Formation
PLANT CELL, June 1, 2004; 16(suppl_1): S190 - S202.
[Full Text] [PDF]

Home page
 Plant CellHome page
F. M. Carland and T. Nelson
COTYLEDON VASCULAR PATTERN2-Mediated Inositol (1,4,5) Triphosphate Signal Transduction Is Essential for Closed Venation Patterns of Arabidopsis Foliar Organs
PLANT CELL, May 1, 2004; 16(5): 1263 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Lalanne, D. Honys, A. Johnson, G. H. H. Borner, K. S. Lilley, P. Dupree, U. Grossniklaus, and D. Twell
SETH1 and SETH2, Two Components of the Glycosylphosphatidylinositol Anchor Biosynthetic Pathway, Are Required for Pollen Germination and Tube Growth in Arabidopsis
PLANT CELL, January 1, 2004; 16(1): 229 - 240.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists