Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online March 28, 2003; 10.1105/tpc.010140

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
15/5/1242    most recent
tpc.010140v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (35)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pimpl, P.
Right arrow Articles by Denecke, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pimpl, P.
Right arrow Articles by Denecke, J.
Agricola
Right arrow Articles by Pimpl, P.
Right arrow Articles by Denecke, J.
The Plant Cell, Vol. 15, 1242-1256, May 2003, Copyright © 2003,
American Society of Plant Biologists

The GTPase ARF1p Controls the Sequence-Specific Vacuolar Sorting Route to the Lytic Vacuole

Peter Pimpl, Sally L. Hanton, J. Philip Taylor, Luis L. Pinto-daSilva and Jürgen Denecke1

Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom

1 To whom correspondence should be addressed. E-mail j.denecke{at}leeds.ac.uk; fax 44-113-3432835

We have studied the transport of soluble cargo molecules by inhibiting specific transport steps to and from the Golgi apparatus. Inhibition of export from the Golgi via coexpression of a dominant-negative GTP-restricted ARF1 mutant (Q71L) inhibits the secretion of {alpha}-amylase and simultaneously induces the secretion of the vacuolar protein phytepsin to the culture medium. By contrast, specific inhibition of endoplasmic reticulum export via overexpression of Sec12p or coexpression of a GTP-restricted form of Sar1p inhibits the anterograde transport of either cargo molecule in a similar manner. Increased secretion of the vacuolar protein was not observed after incubation with the drug brefeldin A or after coexpression of the GDP-restricted mutant of ARF1 (T31N). Therefore, the differential effect of inducing the secretion of one cargo molecule while inhibiting the secretion of another is dependent on the GTP hydrolysis by ARF1p and is not caused by a general inhibition of Golgi-derived COPI vesicle traffic. Moreover, we demonstrate that GTP-restricted ARF1-stimulated secretion is observed only for cargo molecules that are expected to be sorted in a BP80-dependent manner, exhibiting sequence-specific, context-independent, vacuolar sorting signals. Induced secretion of proteins carrying C-terminal vacuolar sorting signals was not observed. This finding suggests that ARF1p influences the BP80-mediated transport route to the vacuole in addition to transport steps of the default secretory pathway to the cell surface.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
I. Hwang
Sorting and Anterograde Trafficking at the Golgi Apparatus
Plant Physiology, October 1, 2008; 148(2): 673 - 683.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. G. Robinson, L. Jiang, and K. Schumacher
The Endosomal System of Plants: Charting New and Familiar Territories
Plant Physiology, August 1, 2008; 147(4): 1482 - 1492.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Rojo and J. Denecke
What Is Moving in the Secretory Pathway of Plants?
Plant Physiology, August 1, 2008; 147(4): 1493 - 1503.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Nielsen, A. Y. Cheung, and T. Ueda
The Regulatory RAB and ARF GTPases for Vesicular Trafficking
Plant Physiology, August 1, 2008; 147(4): 1516 - 1526.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. A. Matheson, S. L. Hanton, M. Rossi, M. Latijnhouwers, G. Stefano, L. Renna, and F. Brandizzi
Multiple Roles of ADP-Ribosylation Factor 1 in Plant Cells Include Spatially Regulated Recruitment of Coatomer and Elements of the Golgi Matrix
Plant Physiology, April 1, 2007; 143(4): 1615 - 1627.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. K. Min, S. J. Kim, Y. Miao, J. Shin, L. Jiang, and I. Hwang
Overexpression of Arabidopsis AGD7 Causes Relocation of Golgi-Localized Proteins to the Endoplasmic Reticulum and Inhibits Protein Trafficking in Plant Cells
Plant Physiology, April 1, 2007; 143(4): 1601 - 1614.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. Jaquinod, F. Villiers, S. Kieffer-Jaquinod, V. Hugouvieux, C. Bruley, J. Garin, and J. Bourguignon
A Proteomics Dissection of Arabidopsis thaliana Vacuoles Isolated from Cell Culture
Mol. Cell. Proteomics, March 1, 2007; 6(3): 394 - 412.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Moreau, F. Brandizzi, S. Hanton, L. Chatre, S. Melser, C. Hawes, and B. Satiat-Jeunemaitre
The plant ER-Golgi interface: a highly structured and dynamic membrane complex
J. Exp. Bot., January 1, 2007; 58(1): 49 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
O. Foresti, L. L.P. daSilva, and J. Denecke
Overexpression of the Arabidopsis Syntaxin PEP12/SYP21 Inhibits Transport from the Prevacuolar Compartment to the Lytic Vacuole in Vivo
PLANT CELL, September 1, 2006; 18(9): 2275 - 2293.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, O. Foresti, and J. Denecke
Targeting of the Plant Vacuolar Sorting Receptor BP80 Is Dependent on Multiple Sorting Signals in the Cytosolic Tail
PLANT CELL, June 1, 2006; 18(6): 1477 - 1497.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Oliviusson, O. Heinzerling, S. Hillmer, G. Hinz, Y. C. Tse, L. Jiang, and D. G. Robinson
Plant Retromer, Localized to the Prevacuolar Compartment and Microvesicles in Arabidopsis, May Interact with Vacuolar Sorting Receptors
PLANT CELL, May 1, 2006; 18(5): 1239 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Maruyama, L. C. Mun, M. Tatsuhara, M. Sawada, M. Ishimoto, and S. Utsumi
Multiple Vacuolar Sorting Determinants Exist in Soybean 11S Globulin
PLANT CELL, May 1, 2006; 18(5): 1253 - 1273.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Pimpl, J. P. Taylor, C. Snowden, S. Hillmer, D. G. Robinson, and J. Denecke
Golgi-Mediated Vacuolar Sorting of the Endoplasmic Reticulum Chaperone BiP May Play an Active Role in Quality Control within the Secretory Pathway
PLANT CELL, January 1, 2006; 18(1): 198 - 211.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. K. Gebbie, J. E. Burn, C. H. Hocart, and R. E. Williamson
Genes encoding ADP-ribosylation factors in Arabidopsis thaliana L. Heyn.; genome analysis and antisense suppression
J. Exp. Bot., April 1, 2005; 56(414): 1079 - 1091.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Xu and B. Scheres
Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 Function in Epidermal Cell Polarity
PLANT CELL, February 1, 2005; 17(2): 525 - 536.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, J. P. Taylor, J. L. Hadlington, S. L. Hanton, C. J. Snowden, S. J. Fox, O. Foresti, F. Brandizzi, and J. Denecke
Receptor Salvage from the Prevacuolar Compartment Is Essential for Efficient Vacuolar Protein Targeting
PLANT CELL, January 1, 2005; 17(1): 132 - 148.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, E. L. Snapp, J. Denecke, J. Lippincott-Schwartz, C. Hawes, and F. Brandizzi
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as Single Mobile Secretory Units in Plant Cells
PLANT CELL, July 1, 2004; 16(7): 1753 - 1771.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists