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Disruption of the AtREV3 Gene Causes Hypersensitivity to Ultraviolet B Light and -Rays in Arabidopsis: Implication of the Presence of a Translesion Synthesis Mechanism in Plants

^a Department of Ion-Beam-Applied Biology, Japan Atomic Energy Research Institute, Takasaki 370-1292, Japan
^b Department of Biology, Hanoi University of Science, Hanoi, Vietnam
^c Faculty of Pharmaceutical Science, Kanazawa University, Kanazawa, Ishikawa 920-0934, Japan

² To whom correspondence should be addressed. E-mail sakamoto{at}taka.jaeri.go.jp; fax 81-27-346-9688

To investigate UV light response mechanisms in higher plants, we isolated a UV light–sensitive mutant, rev3-1, in Arabidopsis. The root growth of rev3-1 was inhibited after UV-B irradiation under both light and dark conditions. We found that chromosome 1 of rev3-1 was broken at a minimum of three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase (AtREV3), which is thought to be involved in translesion synthesis. The rev3-1 seedlings also were sensitive to -rays and mitomycin C, which are known to inhibit DNA replication. Incorporation of bromodeoxyuridine after UV-B irradiation was less in rev3-1 than in the wild type. These results indicate that UV light–damaged DNA interrupted DNA replication in the rev3-1 mutant, leading to the inhibition of cell division and root elongation.

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