Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online May 13, 2005; 10.1105/tpc.105.031393

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
17/6/1685    most recent
tpc.105.031393v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Plant Cell
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, X.
Right arrow Articles by Clouse, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, X.
Right arrow Articles by Clouse, S. D.
Agricola
Right arrow Articles by Wang, X.
Right arrow Articles by Clouse, S. D.
The Plant Cell 17:1685-1703 (2005)
© 2005 American Society of Plant Biologists

Identification and Functional Analysis of in Vivo Phosphorylation Sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 Receptor Kinase

Xiaofeng Wanga, Michael B. Gosheb, Erik J. Soderblomb, Brett S. Phinneyc, Jason A. Kucharc, Jia Lid, Tadao Asamie, Shigeo Yoshidae, Steven C. Huberf and Steven D. Clousea,1

a Department of Horticultural Science, North Carolina State University, Raleigh, North Carolina 27695-7609
b Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695-7622
c Proteomics Facility, Michigan State University, East Lansing, Michigan 48824
d Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019-0245
e RIKEN, Institute of Physical and Chemical Research, Wako, Saitama, 351-0198 Japan
f U.S. Department of Agriculture, Agricultural Research Service, University of Illinois, Urbana, Illinois 61801-3838

1 To whom correspondence should be addressed. E-mail steve_clouse{at}ncsu.edu; fax 919-515-2505.

Brassinosteroids (BRs) regulate multiple aspects of plant growth and development and require an active BRASSINOSTEROID-INSENSITIVE1 (BRI1) and BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) for hormone perception and signal transduction. Many animal receptor kinases exhibit ligand-dependent oligomerization followed by autophosphorylation and activation of the intracellular kinase domain. To determine if early events in BR signaling share this mechanism, we used coimmunoprecipitation of epitope-tagged proteins to show that in vivo association of BRI1 and BAK1 was affected by endogenous and exogenous BR levels and that phosphorylation of both BRI1 and BAK1 on Thr residues was BR dependent. Immunoprecipitation of epitope-tagged BRI1 from Arabidopsis thaliana followed by liquid chromatography–tandem mass spectrometry (LC/MS/MS) identified S-838, S-858, T-872, and T-880 in the juxtamembrane region, T-982 in the kinase domain, and S-1168 in C-terminal region as in vivo phosphorylation sites of BRI1. MS analysis also strongly suggested that an additional two residues in the juxtamembrane region and three sites in the activation loop of kinase subdomain VII/VIII were phosphorylated in vivo. We also identified four specific BAK1 autophosphorylation sites in vitro using LC/MS/MS. Site-directed mutagenesis of identified and predicted BRI1 phosphorylation sites revealed that the highly conserved activation loop residue T-1049 and either S-1044 or T-1045 were essential for kinase function in vitro and normal BRI1 signaling in planta. Mutations in the juxtamembrane or C-terminal regions had only small observable effects on autophosphorylation and in planta signaling but dramatically affected phosphorylation of a peptide substrate in vitro. These findings are consistent with many aspects of the animal receptor kinase model in which ligand-dependent autophosphorylation of the activation loop generates a functional kinase, whereas phosphorylation of noncatalytic intracellular domains is required for recognition and/or phosphorylation of downstream substrates.


Related articles in Plant Cell:

Brassinosteroid Perception and Signaling: Heterodimerization and Phosphorylation of Receptor-Like Kinases BRI1 and BAK1
Nancy A. Eckardt
Plant Cell 2005 17: 1638-1640. [Full Text]  



This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Miyahara, T. A. Hirani, M. Oakes, A. Kereszt, B. Kobe, M. A. Djordjevic, and P. M. Gresshoff
Soybean Nodule Autoregulation Receptor Kinase Phosphorylates Two Kinase-associated Protein Phosphatases in Vitro
J. Biol. Chem., September 12, 2008; 283(37): 25381 - 25391.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Albrecht, E. Russinova, B. Kemmerling, M. Kwaaitaal, and S. C. de Vries
Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE Proteins Serve Brassinosteroid-Dependent and -Independent Signaling Pathways
Plant Physiology, September 1, 2008; 148(1): 611 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Aker and S. C. de Vries
Plasma Membrane Receptor Complexes
Plant Physiology, August 1, 2008; 147(4): 1560 - 1564.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Geldner and S. Robatzek
Plant Receptors Go Endosomal: A Moving View on Signal Transduction
Plant Physiology, August 1, 2008; 147(4): 1565 - 1574.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. J. Groen, S. C. de Vries, and K. S. Lilley
A Proteomics Approach to Membrane Trafficking
Plant Physiology, August 1, 2008; 147(4): 1584 - 1589.
[Full Text] [PDF]

Home page
 ScienceHome page
W. Tang, T.-W. Kim, J. A. Oses-Prieto, Y. Sun, Z. Deng, S. Zhu, R. Wang, A. L. Burlingame, and Z.-Y. Wang
BSKs Mediate Signal Transduction from the Receptor Kinase BRI1 in Arabidopsis
Science, July 25, 2008; 321(5888): 557 - 560.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. AbuQamar, M.-F. Chai, H. Luo, F. Song, and T. Mengiste
Tomato Protein Kinase 1b Mediates Signaling of Plant Responses to Necrotrophic Fungi and Insect Herbivory
PLANT CELL, July 1, 2008; 20(7): 1964 - 1983.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Yu, L. Li, L. Li, M. Guo, J. Chory, and Y. Yin
From the Cover: Modulation of brassinosteroid-regulated gene expression by jumonji domain-containing proteins ELF6 and REF6 in Arabidopsis
PNAS, May 27, 2008; 105(21): 7618 - 7623.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W. Tang, Z. Deng, J. A. Oses-Prieto, N. Suzuki, S. Zhu, X. Zhang, A. L. Burlingame, and Z.-Y. Wang
Proteomics Studies of Brassinosteroid Signal Transduction Using Prefractionation and Two-dimensional DIGE
Mol. Cell. Proteomics, April 1, 2008; 7(4): 728 - 738.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Jia, X. Liu, H. A. Owen, and D. Zhao
Signaling of cell fate determination by the TPD1 small protein and EMS1 receptor kinase
PNAS, February 12, 2008; 105(6): 2220 - 2225.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. L. Heazlewood, P. Durek, J. Hummel, J. Selbig, W. Weckwerth, D. Walther, and W. X. Schulze
PhosPhAt: a database of phosphorylation sites in Arabidopsis thaliana and a plant-specific phosphorylation site predictor
Nucleic Acids Res., January 11, 2008; 36(suppl_1): D1015 - D1021.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. S. Ali, K. V. S. K. Prasad, I. Day, and A. S. N. Reddy
Ligand-Dependent Reduction in the Membrane Mobility of FLAGELLIN SENSITIVE2, an Arabidopsis Receptor-Like Kinase
Plant Cell Physiol., November 1, 2007; 48(11): 1601 - 1611.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Castells and J. M. Casacuberta
Signalling through kinase-defective domains: the prevalence of atypical receptor-like kinases in plants
J. Exp. Bot., October 20, 2007; (2007) erm226v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
T. Niittyla, A. T. Fuglsang, M. G. Palmgren, W. B. Frommer, and W. X. Schulze
Temporal Analysis of Sucrose-induced Phosphorylation Changes in Plasma Membrane Proteins of Arabidopsis
Mol. Cell. Proteomics, October 1, 2007; 6(10): 1711 - 1726.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. A. Domagalska, F. M. Schomburg, R. M. Amasino, R. D. Vierstra, F. Nagy, and S. J. Davis
Attenuation of brassinosteroid signaling enhances FLC expression and delays flowering
Development, August 1, 2007; 134(15): 2841 - 2850.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Heese, D. R. Hann, S. Gimenez-Ibanez, A. M. E. Jones, K. He, J. Li, J. I. Schroeder, S. C. Peck, and J. P. Rathjen
The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants
PNAS, July 17, 2007; 104(29): 12217 - 12222.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
N. Geldner, D. L. Hyman, X. Wang, K. Schumacher, and J. Chory
Endosomal signaling of plant steroid receptor kinase BRI1
Genes & Dev., July 1, 2007; 21(13): 1598 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Shin, S. Alvarez, A. Y. Burch, J. M. Jez, and D. P. Schachtman
Phosphoproteomic identification of targets of the Arabidopsis sucrose nonfermenting-like kinase SnRK2.8 reveals a connection to metabolic processes
PNAS, April 10, 2007; 104(15): 6460 - 6465.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Ouelhadj, M. Kaminski, M. Mittag, and K. Humbeck
Receptor-like protein kinase HvLysMR1 of barley (Hordeum vulgare L.) is induced during leaf senescence and heavy metal stress
J. Exp. Bot., April 1, 2007; 58(6): 1381 - 1396.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Y. Belkhadir and J. Chory
Brassinosteroid Signaling: A Paradigm for Steroid Hormone Signaling from the Cell Surface
Science, December 1, 2006; 314(5804): 1410 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
R. Karlova and S. C. de Vries
Advances in Understanding Brassinosteroid Signaling
Sci. Signal., September 26, 2006; 2006(354): pe36 - pe36.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
X. Wang and J. Chory
Brassinosteroids Regulate Dissociation of BKI1, a Negative Regulator of BRI1 Signaling, from the Plasma Membrane
Science, August 25, 2006; 313(5790): 1118 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
B. Chow and P. McCourt
Plant hormone receptors: perception is everything
Genes & Dev., August 1, 2006; 20(15): 1998 - 2008.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Robatzek, D. Chinchilla, and T. Boller
Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis.
Genes & Dev., March 1, 2006; 20(5): 537 - 542.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Karlova, S. Boeren, E. Russinova, J. Aker, J. Vervoort, and S. de Vries
The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 Protein Complex Includes BRASSINOSTEROID-INSENSITIVE1
PLANT CELL, March 1, 2006; 18(3): 626 - 638.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Nakamura, S. Fujioka, H. Sunohara, N. Kamiya, Z. Hong, Y. Inukai, K. Miura, S. Takatsuto, S. Yoshida, M. Ueguchi-Tanaka, et al.
The Role of OsBRI1 and Its Homologous Genes, OsBRL1 and OsBRL3, in Rice
Plant Physiology, February 1, 2006; 140(2): 580 - 590.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Colcombet, A. Boisson-Dernier, R. Ros-Palau, C. E. Vera, and J. I. Schroeder
Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASES1 and 2 Are Essential for Tapetum Development and Microspore Maturation
PLANT CELL, December 1, 2005; 17(12): 3350 - 3361.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Brassinosteroid Perception and Signaling: Heterodimerization and Phosphorylation of Receptor-Like Kinases BRI1 and BAK1
PLANT CELL, June 1, 2005; 17(6): 1638 - 1640.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2005 by the American Society of Plant Biologists