This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 17/6/1737    most recent tpc.105.032615v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Chan, J. Articles by Lloyd, C. Search for Related Content PubMed PubMed Citation Articles by Chan, J. Articles by Lloyd, C. Agricola Articles by Chan, J. Articles by Lloyd, C.

Localization of the Microtubule End Binding Protein EB1 Reveals Alternative Pathways of Spindle Development in Arabidopsis Suspension Cells

Department of Cell and Developmental Biology, John Innes Centre, Norwich NR 4 7UH, United Kingdom

² To whom correspondence should be addressed. E-mail jordi.chan{at}bbsrc.ac.uk; fax 44-1603-450022.

In a previous study on Arabidopsis thaliana suspension cells transiently infected with the microtubule end binding protein AtEB1a–green fluorescent protein (GFP), we reported that interphase microtubules grow from multiple sites dispersed over the cortex, with plus ends forming the characteristic comet-like pattern. In this study, AtEB1a-GFP was used to study the transitions of microtubule arrays throughout the division cycle of cells lacking a defined centrosome. During division, the dispersed origin of microtubules was replaced by a more focused pattern with the plus end comets growing away from sites associated with the nuclear periphery. The mitotic spindle then evolved in two quite distinct ways depending on the presence or absence of the preprophase band (PPB): the cells displaying outside-in as well as inside-out mitotic pathways. In those cells possessing a PPB, the fusion protein labeled material at the nuclear periphery that segregated into two polar caps, perpendicular to the PPB, before nuclear envelope breakdown (NEBD). These polar caps then marked the spindle poles upon NEBD. However, in the population of cells without PPBs, there was no prepolarization of material at the nuclear envelope before NEBD, and the bipolar spindle only emerged clearly after NEBD. Such cells had variable spindle orientations and enhanced phragmoplast mobility, suggesting that the PPB is involved in a polarization event that promotes early spindle pole morphogenesis and subsequent positional stability during division. Astral-like microtubules are not usually prominent in plant cells, but they are clearly seen in these Arabidopsis cells, and we hypothesize that they may be involved in orienting the division plane, particularly where the plane is not determined before division.

This article has been cited by other articles:

				J. C. Ambrose and R. Cyr Mitotic Spindle Organization by the Preprophase Band Mol Plant, September 26, 2008; (2008) ssn054v1. [Abstract] [Full Text] [PDF]

				S. R. Bisgrove, Y.-R. J. Lee, B. Liu, N. T. Peters, and D. L. Kropf The Microtubule Plus-End Binding Protein EB1 Functions in Root Responses to Touch and Gravity Signals in Arabidopsis PLANT CELL, February 1, 2008; 20(2): 396 - 410. [Abstract] [Full Text] [PDF]

				V. Kirik, U. Herrmann, C. Parupalli, J. C. Sedbrook, D. W. Ehrhardt, and M. Hulskamp CLASP localizes in two discrete patterns on cortical microtubules and is required for cell morphogenesis and cell division in Arabidopsis J. Cell Sci., December 15, 2007; 120(24): 4416 - 4425. [Abstract] [Full Text] [PDF]

				T. Hayashi, T. Sano, N. Kutsuna, F. Kumagai-Sano, and S. Hasezawa Contribution of Anaphase B to Chromosome Separation in Higher Plant Cells Estimated by Image Processing Plant Cell Physiol., October 1, 2007; 48(10): 1509 - 1513. [Abstract] [Full Text] [PDF]

				T. Desprez, M. Juraniec, E. F. Crowell, H. Jouy, Z. Pochylova, F. Parcy, H. Hofte, M. Gonneau, and S. Vernhettes Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thaliana PNAS, September 25, 2007; 104(39): 15572 - 15577. [Abstract] [Full Text] [PDF]

				B. Menand, G. Calder, and L. Dolan Both chloronemal and caulonemal cells expand by tip growth in the moss Physcomitrella patens J. Exp. Bot., May 1, 2007; 58(7): 1843 - 1849. [Abstract] [Full Text] [PDF]

				J. C. Ambrose and R. Cyr The Kinesin ATK5 Functions in Early Spindle Assembly in Arabidopsis PLANT CELL, January 1, 2007; 19(1): 226 - 236. [Abstract] [Full Text] [PDF]

				P. Dhonukshe, N. Vischer, and T. W. J. Gadella Jr Contribution of microtubule growth polarity and flux to spindle assembly and functioning in plant cells. J. Cell Sci., August 1, 2006; 119(Pt 15): 3193 - 3205. [Abstract] [Full Text] [PDF]

				R. Dixit, E. Chang, and R. Cyr Establishment of Polarity during Organization of the Acentrosomal Plant Cortical Microtubule Array Mol. Biol. Cell, March 1, 2006; 17(3): 1298 - 1305. [Abstract] [Full Text] [PDF]