Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on June 6, 2002; 10.1105/tpc.001628

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow All Versions of this Article:
14/6/1377    most recent
tpc.001628v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fan, W.
Right arrow Articles by Dong, X.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Fan, W.
Right arrow Articles by Dong, X.
Agricola
Right arrow Articles by Fan, W.
Right arrow Articles by Dong, X.

Received January 16, 2002
Accepted March 9, 2002

In Vivo Interaction between NPR1 and Transcription Factor TGA2 Leads to Salicylic Acid--Mediated Gene Activation in Arabidopsis

Weihua Fan 1 and Xinnian Dong 1*

1 Developmental, Cell, and Molecular Biology Group, Department of Biology, Duke University, Durham, North Carolina 27708-1000

* To whom correspondence should be addressed. E-mail: xdong{at}duke.edu.

The Arabidopsis NPR1 protein is a key regulator of salicylic acid (SA)--mediated gene expression in systemic acquired resistance. Based on yeast two-hybrid analysis, NPR1 has been suggested to interact with members of the TGA family of transcription factors, including TGA2 (AHBP-1b). However, genetic evidence demonstrating that the NPR1--TGA interaction occurs in planta is still lacking, and the role of this interaction in SA-mediated gene activation has yet to be determined. In this study, we expressed a truncated form of TGA2 in Arabidopsis and found that the resulting transgenic lines displayed phenotypes similar to those of npr1 mutants. This dominant-negative effect of the TGA2 mutant shows that TGA2 and NPR1 interact in planta. We also present biochemical evidence indicating that this interaction is specific and enhanced by SA treatment. Moreover, using a chimera reporter system, we found that a chimeric TGA2GAL4 transcription factor activated a UAS GAL::GUS reporter gene in response to SA and that this activation was abolished in the npr1 mutant. NPR1 is required for the DNA binding activity of the transcription factor. These genetic data clearly demonstrate that TGA2 is a SA-responsive and NPR1-dependent transcription activator.




This article has been cited by other articles:


Home page
 J Exp BotHome page
M.-H. Weech, M. Chapleau, L. Pan, C. Ide, and J. C. Bede
Caterpillar saliva interferes with induced Arabidopsis thaliana defence responses via the systemic acquired resistance pathway
J. Exp. Bot., June 1, 2008; 59(9): 2437 - 2448.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
M. Chern, P. E. Canlas, and P. C. Ronald
Strong Suppression of Systemic Acquired Resistance in Arabidopsis by NRR is Dependent on its Ability to Interact with NPR1and its Putative Repression Domain
Mol Plant, May 1, 2008; 1(3): 552 - 559.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. G. Bakker, M. B. Traw, C. Toomajian, M. Kreitman, and J. Bergelson
Low Levels of Polymorphism in Genes That Control the Activation of Defense Response in Arabidopsis thaliana
Genetics, April 1, 2008; 178(4): 2031 - 2043.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Fabro, J. A. Di Rienzo, C. A. Voigt, T. Savchenko, K. Dehesh, S. Somerville, and M. E. Alvarez
Genome-Wide Expression Profiling Arabidopsis at the Stage of Golovinomyces cichoracearum Haustorium Formation
Plant Physiology, March 1, 2008; 146(3): 1421 - 1439.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
P. Mao, M. Duan, C. Wei, and Y. Li
WRKY62 Transcription Factor Acts Downstream of Cytosolic NPR1 and Negatively Regulates Jasmonate-Responsive Gene Expression
Plant Cell Physiol., June 1, 2007; 48(6): 833 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Nobuta, R.A. Okrent, M. Stoutemyer, N. Rodibaugh, L. Kempema, M.C. Wildermuth, and R.W. Innes
The GH3 Acyl Adenylase Family Member PBS3 Regulates Salicylic Acid-Dependent Defense Responses in Arabidopsis
Plant Physiology, June 1, 2007; 144(2): 1144 - 1156.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Kesarwani, J. Yoo, and X. Dong
Genetic Interactions of TGA Transcription Factors in the Regulation of Pathogenesis-Related Genes and Disease Resistance in Arabidopsis
Plant Physiology, May 1, 2007; 144(1): 336 - 346.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Rochon, P. Boyle, T. Wignes, P. R. Fobert, and C. Despres
The Coactivator Function of Arabidopsis NPR1 Requires the Core of Its BTB/POZ Domain and the Oxidation of C-Terminal Cysteines
PLANT CELL, December 1, 2006; 18(12): 3670 - 3685.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B.-K. Ham, J. M. Park, S.-B. Lee, M. J. Kim, I.-J. Lee, K.-J. Kim, C. S. Kwon, and K.-H. Paek
Tobacco Tsip1, a DnaJ-Type Zn Finger Protein, Is Recruited to and Potentiates Tsi1-Mediated Transcriptional Activation
PLANT CELL, August 1, 2006; 18(8): 2005 - 2020.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. J. Heidel and X. Dong
Fitness Benefits of Systemic Acquired Resistance During Hyaloperonospora parasitica Infection in Arabidopsis thaliana
Genetics, July 1, 2006; 173(3): 1621 - 1628.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Yaeno, B. Saito, T. Katsuki, and K. Iba
Ozone-induced Expression of the Arabidopsis FAD7 Gene Requires Salicylic Acid, but not NPR1 and SID2
Plant Cell Physiol., March 1, 2006; 47(3): 355 - 362.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. A.J. Mur, P. Kenton, R. Atzorn, O. Miersch, and C. Wasternack
The Outcomes of Concentration-Specific Interactions between Salicylate and Jasmonate Signaling Include Synergy, Antagonism, and Oxidative Stress Leading to Cell Death
Plant Physiology, January 1, 2006; 140(1): 249 - 262.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. R. Weigel, U. M. Pfitzner, and C. Gatz
Interaction of NIMIN1 with NPR1 Modulates PR Gene Expression in Arabidopsis
PLANT CELL, April 1, 2005; 17(4): 1279 - 1291.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. J. Wisser, Q. Sun, S. H. Hulbert, S. Kresovich, and R. J. Nelson
Identification and Characterization of Regions of the Rice Genome Associated With Broad-Spectrum, Quantitative Disease Resistance
Genetics, April 1, 2005; 169(4): 2277 - 2293.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Huang, J. M. Yeakley, E. W. Garcia, J. D. Holdridge, J.-B. Fan, and S. A. Whitham
Salicylic Acid-Dependent Expression of Host Genes in Compatible Arabidopsis-Virus Interactions
Plant Physiology, March 1, 2005; 137(3): 1147 - 1159.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. J. Heidel, J. D. Clarke, J. Antonovics, and X. Dong
Fitness Costs of Mutations Affecting the Systemic Acquired Resistance Pathway in Arabidopsis thaliana
Genetics, December 1, 2004; 168(4): 2197 - 2206.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. M. Scott, S. M. Clarke, J. E. Wood, and L. A.J. Mur
Salicylate Accumulation Inhibits Growth at Chilling Temperature in Arabidopsis
Plant Physiology, June 1, 2004; 135(2): 1040 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Li, G. Brader, and E. T. Palva
The WRKY70 Transcription Factor: A Node of Convergence for Jasmonate-Mediated and Salicylate-Mediated Signals in Plant Defense
PLANT CELL, February 1, 2004; 16(2): 319 - 331.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Hugot, M.-P. Riviere, C. Moreilhon, M. A. Dayem, J. Cozzitorto, G. Arbiol, P. Barbry, C. Weiss, and E. Galiana
Coordinated Regulation of Genes for Secretion in Tobacco at Late Developmental Stages: Association with Resistance against Oomycetes
Plant Physiology, February 1, 2004; 134(2): 858 - 870.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Zhang, M. J. Tessaro, M. Lassner, and X. Li
Knockout Analysis of Arabidopsis Transcription Factors TGA2, TGA5, and TGA6 Reveals Their Redundant and Essential Roles in Systemic Acquired Resistance
PLANT CELL, November 1, 2003; 15(11): 2647 - 2653.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Lu, D. N. Rate, J. T. Song, and J. T. Greenberg
ACD6, a Novel Ankyrin Protein, Is a Regulator and an Effector of Salicylic Acid Signaling in the Arabidopsis Defense Response
PLANT CELL, October 1, 2003; 15(10): 2408 - 2420.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
A New Twist on Systemic Acquired Resistance: Redox Control of the NPR1-TGA1 Interaction by Salicylic Acid
PLANT CELL, September 1, 2003; 15(9): 1947 - 1949.
[Full Text] [PDF]

Home page
 Plant CellHome page
C. Despres, C. Chubak, A. Rochon, R. Clark, T. Bethune, D. Desveaux, and P. R. Fobert
The Arabidopsis NPR1 Disease Resistance Protein Is a Novel Cofactor That Confers Redox Regulation of DNA Binding Activity to the Basic Domain/Leucine Zipper Transcription Factor TGA1
PLANT CELL, September 1, 2003; 15(9): 2181 - 2191.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Johnson, E. Boden, and J. Arias
Salicylic Acid and NPR1 Induce the Recruitment of trans-Activating TGA Factors to a Defense Gene Promoter in Arabidopsis
PLANT CELL, August 1, 2003; 15(8): 1846 - 1858.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. H. Spoel, A. Koornneef, S. M. C. Claessens, J. P. Korzelius, J. A. Van Pelt, M. J. Mueller, A. J. Buchala, J.-P. Metraux, R. Brown, K. Kazan, et al.
NPR1 Modulates Cross-Talk between Salicylate- and Jasmonate-Dependent Defense Pathways through a Novel Function in the Cytosol
PLANT CELL, March 1, 2003; 15(3): 760 - 770.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Alabadi, A. Devoto, and N. A. Eckardt
Arabidopsis Research Heats Up in Seville
PLANT CELL, September 1, 2002; 14(9): 1987 - 1994.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2002 by the American Society of Plant Biologists