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Plant Cell Advance Online Publication
Published on September 26, 2002; 10.1105/tpc.005207

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Received June 10, 2002
Accepted July 28, 2002

Rose Scent: Genomics Approach to Discovering Novel Floral Fragrance-Related Genes

Inna Guterman 1, Moshe Shalit 2, Naama Menda 1, Dan Piestun 1, Mery Dafny-Yelin 1, Gil Shalev 1, Einat Bar 3, Olga Davydov 4, Mariana Ovadis 1, Michal Emanuel 1, Jihong Wang 5, Zach Adam 1, Eran Pichersky 5, Efraim Lewinsohn 3, Dani Zamir 1, Alexander Vainstein 1, and David Weiss 1*

1 Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
2 Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel; Division of Aromatic Plants, Agricultural Research Organization, Newe Ya'ar, P.O. Box 1021, Ramat Yishay 30095, Israel
3 Division of Aromatic Plants, Agricultural Research Organization, Newe Ya'ar, P.O. Box 1021, Ramat Yishay 30095, Israel
4 Department of Plant Sciences, Weizmann Institute of Science, P.O. Box 26, Rehovot 76100, Israel
5 Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048

* To whom correspondence should be addressed. E-mail: weiss{at}agri.huji.ac.il.

For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of ~2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants.






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