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Plant Cell Advance Online Publication
Published on October 15, 2002; 10.1105/tpc.006262

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Received July 11, 2002
Accepted September 1, 2002

A Two-Component Mn2+-Sensing System Negatively Regulates Expression of the mntCAB Operon in Synechocystis

Katsushi Yamaguchi 1, Iwane Suzuki 2, Hiroshi Yamamoto 1, Alexander Lyukevich 3, Irina Bodrova 4, Dmitry A. Los 3, Irina Piven 5, Vladislav Zinchenko 5, Minoru Kanehisa 6, and Norio Murata 2*

1 Department of Regulation Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
2 Department of Regulation Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Molecular Biomechanics, School of Life Science, Graduate University of Advanced Studies, Okazaki 444-8585, Japan
3 Department of Regulation Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia
4 Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia
5 Department of Genetics, Moscow State University, Moscow 119899, Russia
6 Institute for Chemical Research, Kyoto University, Uji 611-0011, Japan

* To whom correspondence should be addressed. E-mail: murata{at}nibb.ac.jp.

Mn is an essential component of the oxygen-evolving machinery of photosynthesis and is an essential cofactor of several important enzymes, such as Mn-superoxide dismutase and Mn-catalase. The availability of Mn in the environment varies, and little is known about the mechanisms for maintaining cytoplasmic Mn2+ ion homeostasis. Using a DNA microarray, we screened knockout libraries of His kinases and response regulators of Synechocystis sp PCC 6803 to identify possible participants in this process. We identified a His kinase, ManS, which might sense the extracellular concentration of Mn2+ ions, and a response regulator, ManR, which might regulate the expression of the mntCAB operon for the ABC-type transporter of Mn2+ ions. Furthermore, analysis with the DNA microarray and by reverse transcription PCR suggested that ManS produces a signal that activates ManR, which represses the expression of the mntCAB operon. At low concentrations of Mn2+ ions, ManS does not generate a signal, with resulting inactivation of ManR and subsequent expression of the mntCAB operon.






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