Plant Cell Illumina, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on January 17, 2003; 10.1105/tpc.006882

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow All Versions of this Article:
15/2/545    most recent
tpc.006882v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chae, H. S.
Right arrow Articles by Kieber, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chae, H. S.
Right arrow Articles by Kieber, J. J.
Agricola
Right arrow Articles by Chae, H. S.
Right arrow Articles by Kieber, J. J.

Received August 2, 2002
Accepted November 13, 2002

The eto1, eto2, and eto3 Mutations and Cytokinin Treatment Increase Ethylene Biosynthesis in Arabidopsis by Increasing the Stability of ACS Protein

Hyun Sook Chae 1, Francois Faure 1, and Joseph J. Kieber 1*

1 Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280

* To whom correspondence should be addressed. E-mail: jkieber{at}unc.edu.

The Arabidopsis ethylene-overproducing mutants eto1, eto2, and eto3 have been suggested to affect the post-transcriptional regulation of 1-aminocyclopropane-1-carboxylic acid synthase (ACS). Here, we present the positional cloning of the gene corresponding to the dominant eto3 mutation and show that the eto3 phenotype is the result of a missense mutation within the C-terminal domain of ACS9, which encodes one isoform of the Arabidopsis ACS gene family. This mutation is analogous to the dominant eto2 mutation that affects the C-terminal domain of the highly similar ACS5. Analysis of purified recombinant ACS5 and epitope-tagged ACS5 in transgenic Arabidopsis revealed that eto2 does not increase the specific activity of the enzyme either in vitro or in vivo; rather, it increases the half-life of the protein. In a similar manner, cytokinin treatment increased the stability of ACS5 by a mechanism that is at least partially independent of the eto2 mutation. The eto1 mutation was found to act by increasing the function of ACS5 by stabilizing this protein. These results suggest that an important mechanism by which ethylene biosynthesis is controlled is the regulation of the stability of ACS, mediated at least in part through the C-terminal domain.




This article has been cited by other articles:


Home page
 J Exp BotHome page
Z. Lin, S. Zhong, and D. Grierson
Recent advances in ethylene research
J. Exp. Bot., August 1, 2009; 60(12): 3311 - 3336.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Zhang, H. Zhang, R. Quan, X.-C. Wang, and R. Huang
Transcriptional Regulation of the Ethylene Response Factor LeERF2 in the Expression of Ethylene Biosynthesis Genes Controls Ethylene Production in Tomato and Tobacco
Plant Physiology, May 1, 2009; 150(1): 365 - 377.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Ruzicka, M. Simaskova, J. Duclercq, J. Petrasek, E. Zazimalova, S. Simon, J. Friml, M. C. E. Van Montagu, and E. Benkova
Cytokinin regulates root meristem activity via modulation of the polar auxin transport
PNAS, March 17, 2009; 106(11): 4284 - 4289.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Pernisova, P. Klima, J. Horak, M. Valkova, J. Malbeck, P. Soucek, P. Reichman, K. Hoyerova, J. Dubova, J. Friml, et al.
From the Cover: Cytokinins modulate auxin-induced organogenesis in plants via regulation of the auxin efflux
PNAS, March 3, 2009; 106(9): 3609 - 3614.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-Y. Jung, R. Shin, and D. P. Schachtman
Ethylene Mediates Response and Tolerance to Potassium Deprivation in Arabidopsis
PLANT CELL, February 1, 2009; 21(2): 607 - 621.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S.-L. Xu, A. Rahman, T. I. Baskin, and J. J. Kieber
Two Leucine-Rich Repeat Receptor Kinases Mediate Signaling, Linking Cell Wall Biosynthesis and ACC Synthase in Arabidopsis
PLANT CELL, November 1, 2008; 20(11): 3065 - 3079.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Xu, Y. Li, Y. Wang, H. Liu, L. Lei, H. Yang, G. Liu, and D. Ren
Activation of MAPK Kinase 9 Induces Ethylene and Camalexin Biosynthesis and Enhances Sensitivity to Salt Stress in Arabidopsis
J. Biol. Chem., October 3, 2008; 283(40): 26996 - 27006.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. El-Sharkawy, W. S. Kim, S. Jayasankar, A. M. Svircev, and D. C. W. Brown
Differential regulation of four members of the ACC synthase gene family in plum
J. Exp. Bot., May 1, 2008; 59(8): 2009 - 2027.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. Stout, E. Romero-Severson, M. O. Ruegger, and C. Chapple
Semidominant Mutations in Reduced Epidermal Fluorescence 4 Reduce Phenylpropanoid Content in Arabidopsis
Genetics, April 1, 2008; 178(4): 2237 - 2251.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
A. Hahn, R. Zimmermann, D. Wanke, K. Harter, and H. G. Edelmann
The Root Cap Determines Ethylene-Dependent Growth and Development in Maize Roots
Mol Plant, March 1, 2008; 1(2): 359 - 367.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Dugardeyn, F. Vandenbussche, and D. Van Der Straeten
To grow or not to grow: what can we learn on ethylene-gibberellin cross-talk by in silico gene expression analysis?
J. Exp. Bot., January 1, 2008; 59(1): 1 - 16.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Dong, J. L. Bufford, Y. Oono, K. Church, M. Q. Dau, K. Michels, M. Haughton, and G. Tallman
Heat Suppresses Activation of an Auxin-Responsive Promoter in Cultured Guard Cell Protoplasts of Tree Tobacco
Plant Physiology, October 1, 2007; 145(2): 367 - 377.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Bouchez, C. Huard, S. Lorrain, D. Roby, and C. Balague
Ethylene Is One of the Key Elements for Cell Death and Defense Response Control in the Arabidopsis Lesion Mimic Mutant vad1
Plant Physiology, October 1, 2007; 145(2): 465 - 477.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Aki, M. Konishi, T. Kikuchi, T. Fujimori, T. Yoneyama, and S. Yanagisawa
Distinct modulations of the hexokinase1-mediated glucose response and hexokinase1-independent processes by HYS1/CPR5 in Arabidopsis
J. Exp. Bot., September 6, 2007; (2007) erm169v2.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
O. Ortega-Martinez, M. Pernas, R. J. Carol, and L. Dolan
Ethylene Modulates Stem Cell Division in the Arabidopsis thaliana Root
Science, July 27, 2007; 317(5837): 507 - 510.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Ruzicka, K. Ljung, S. Vanneste, R. Podhorska, T. Beeckman, J. Friml, and E. Benkova
Ethylene Regulates Root Growth through Effects on Auxin Biosynthesis and Transport-Dependent Auxin Distribution
PLANT CELL, July 1, 2007; 19(7): 2197 - 2212.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
K. Dreher and J. Callis
Ubiquitin, Hormones and Biotic Stress in Plants
Ann. Bot., May 1, 2007; 99(5): 787 - 822.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Hirose, N. Makita, M. Kojima, T. Kamada-Nobusada, and H. Sakakibara
Overexpression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism
Plant Cell Physiol., March 1, 2007; 48(3): 523 - 539.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. M. Chilley, S. A. Casson, P. Tarkowski, N. Hawkins, K. L.-C. Wang, P. J. Hussey, M. Beale, J. R. Ecker, G. K. Sandberg, and K. Lindsey
The POLARIS Peptide of Arabidopsis Regulates Auxin Transport and Root Growth via Effects on Ethylene Signaling
PLANT CELL, November 1, 2006; 18(11): 3058 - 3072.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. R. Knopf and T. Trebitsh
The Female-Specific Cs-ACS1G Gene of Cucumber. A Case of Gene Duplication and Recombination between the Non-Sex-Specific 1-Aminocyclopropane-1-Carboxylate Synthase Gene and a Branched-Chain Amino Acid Transaminase Gene
Plant Cell Physiol., September 1, 2006; 47(9): 1217 - 1228.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Tanaka, T. Sano, M. Tamaoki, N. Nakajima, N. Kondo, and S. Hasezawa
Cytokinin and auxin inhibit abscisic acid-induced stomatal closure by enhancing ethylene production in Arabidopsis
J. Exp. Bot., July 1, 2006; 57(10): 2259 - 2266.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. A. Samuel, A. Walia, S. D. Mansfield, and B. E. Ellis
Overexpression of SIPK in tobacco enhances ozone-induced ethylene formation and blocks ozone-induced SA accumulation
J. Exp. Bot., August 1, 2005; 56(418): 2195 - 2201.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Y.L. Yuen, J. C. Sedbrook, R. M. Perrin, K. L. Carroll, and P. H. Masson
Loss-of-Function Mutations of ROOT HAIR DEFECTIVE3 Suppress Root Waving, Skewing, and Epidermal Cell File Rotation in Arabidopsis
Plant Physiology, June 1, 2005; 138(2): 701 - 714.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
Y.-F. CHEN, N. ETHERIDGE, and G. E. SCHALLER
Ethylene Signal Transduction
Ann. Bot., May 1, 2005; 95(6): 901 - 915.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. Rieu, S. M. Cristescu, F. J. M. Harren, W. Huibers, L. A. C. J. Voesenek, C. Mariani, and W. H. Vriezen
RP-ACS1, a flooding-induced 1-aminocyclopropane-1-carboxylate synthase gene of Rumex palustris, is involved in rhythmic ethylene production
J. Exp. Bot., March 1, 2005; 56(413): 841 - 849.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. N. Wang, M.-C. Shih, and N. Li
The GUS reporter-aided analysis of the promoter activities of Arabidopsis ACC synthase genes AtACS4, AtACS5, and AtACS7 induced by hormones and stresses
J. Exp. Bot., March 1, 2005; 56(413): 909 - 920.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
Y. SAITO, S. YAMASAKI, N. FUJII, and H. TAKAHASHI
Possible Involvement of CS-ACS1 and Ethylene in Auxin-induced Peg Formation of Cucumber Seedlings
Ann. Bot., February 1, 2005; 95(3): 413 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Liu and S. Zhang
Phosphorylation of 1-Aminocyclopropane-1-Carboxylic Acid Synthase by MPK6, a Stress-Responsive Mitogen-Activated Protein Kinase, Induces Ethylene Biosynthesis in Arabidopsis
PLANT CELL, December 1, 2004; 16(12): 3386 - 3399.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. C. Thain, F. Vandenbussche, L. J.J. Laarhoven, M. J. Dowson-Day, Z.-Y. Wang, E. M. Tobin, F. J.M. Harren, A. J. Millar, and D. Van Der Straeten
Circadian Rhythms of Ethylene Emission in Arabidopsis
Plant Physiology, November 1, 2004; 136(3): 3751 - 3761.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Tsuchisaka and A. Theologis
Unique and Overlapping Expression Patterns among the Arabidopsis 1-Amino-Cyclopropane-1-Carboxylate Synthase Gene Family Members
Plant Physiology, October 1, 2004; 136(2): 2982 - 3000.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Bostick, S. R. Lochhead, A. Honda, S. Palmer, and J. Callis
Related to Ubiquitin 1 and 2 Are Redundant and Essential and Regulate Vegetative Growth, Auxin Signaling, and Ethylene Production in Arabidopsis
PLANT CELL, September 1, 2004; 16(9): 2418 - 2432.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Gachon, A. Mingam, and B. Charrier
Real-time PCR: what relevance to plant studies?
J. Exp. Bot., July 1, 2004; 55(402): 1445 - 1454.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Tsuchisaka and A. Theologis
Heterodimeric interactions among the 1-amino-cyclopropane-1-carboxylate synthase polypeptides encoded by the Arabidopsis gene family
PNAS, February 24, 2004; 101(8): 2275 - 2280.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Yamagami, A. Tsuchisaka, K. Yamada, W. F. Haddon, L. A. Harden, and A. Theologis
Biochemical Diversity among the 1-Amino-cyclopropane-1-Carboxylate Synthase Isozymes Encoded by the Arabidopsis Gene Family
J. Biol. Chem., December 5, 2003; 278(49): 49102 - 49112.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Y. Kim, Y. Liu, E. T. Thorne, H. Yang, H. Fukushige, W. Gassmann, D. Hildebrand, R. E. Sharp, and S. Zhang
Activation of a Stress-Responsive Mitogen-Activated Protein Kinase Cascade Induces the Biosynthesis of Ethylene in Plants
PLANT CELL, November 1, 2003; 15(11): 2707 - 2718.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Vandenbussche, W. H. Vriezen, J. Smalle, L. J.J. Laarhoven, F. J.M. Harren, and D. Van Der Straeten
Ethylene and Auxin Control the Arabidopsis Response to Decreased Light Intensity
Plant Physiology, October 1, 2003; 133(2): 517 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. A. Schmelz, J. Engelberth, H. T. Alborn, P. O'Donnell, M. Sammons, H. Toshima, and J. H. Tumlinson III
Simultaneous analysis of phytohormones, phytotoxins, and volatile organic compounds in plants
PNAS, September 2, 2003; 100(18): 10552 - 10557.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists