Plant Cell Advance Online Publication
Published on December 13, 2002; 10.1105/tpc.007096
Received August 15, 2002
Accepted October 5, 2002
The Transcription Factors HvABI5 and HvVP1 Are Required for the Abscisic Acid Induction
of Gene Expression in Barley Aleurone Cells
Jose Casaretto 1 and Tuan-hua David Ho 1*
1
Plant Biology Program, Department of Biology, Washington University, St. Louis, Missouri
63130
* To whom correspondence should be addressed. E-mail: ho{at}biology.wustl.edu.
The abscisic acid (ABA) response promoter complexes (ABRCs) of the HVA1
and HVA22 genes have been shown to confer ABA-induced gene expression in
cereals. A barley basic domain/Leu zipper (bZIP) transcription factor, HvABI5, is
able to recognize ABRCs in vitro in a sequence-specific manner and to transactivate
ABRC- -glucuronidase reporter genes when introduced to barley aleurone
cells via particle bombardment. This transactivation is dependent on the presence
of another transcription factor, HvVP1, and cannot be blocked by the negative regulator
abi1-1. Using the double-stranded RNA interference technique, we show that HvABI5
and HvVP1 are necessary for the ABA induction of gene expression but have no effect
on another hormone-regulated process, the gibberellin-induced and ABA-suppressed
expression of  -amylase. Our work indicates that although other typical plant
bZIP transcription factors may bind ABRCs in vitro, HvABI5 is related to a subfamily
of bZIPs responsible for the ABA induction of gene expression. Furthermore, HvABI5
and HvVP1 are not involved in the ABA suppression of gene expression.
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