Plant Cell Advance Online Publication
Published on February 24, 2003; 10.1105/tpc.008714
Received October 20, 2002
Accepted December 21, 2002
Disease Resistance and Abiotic Stress Tolerance in Rice Are Inversely Modulated by
an Abscisic Acid-Inducible Mitogen-Activated Protein Kinase
Lizhong Xiong 1 and Yinong Yang 1*
1
Department of Plant Pathology and Program in Cell and Molecular Biology, University
of Arkansas, Fayetteville, Arkansas 72701
* To whom correspondence should be addressed. E-mail: yiyang{at}uark.edu.
Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating
stress responses in eukaryotic organisms. However, little is known about the role
of MAPKs in modulating the interaction of defense pathways activated by biotic and
abiotic factors. In this study, we have isolated and functionally characterized a
stress-responsive MAPK gene (OsMAPK5) from rice. OsMAPK5 is a single-copy
gene but can generate at least two differentially spliced transcripts. The OsMAPK5
gene, its protein, and kinase activity were inducible by abscisic acid as well
as various biotic (pathogen infection) and abiotic (wounding, drought, salt, and
cold) stresses. To determine its biological function, we generated and analyzed transgenic
rice plants with overexpression (using the 35S promoter of Cauliflower mosaic
virus) or suppression (using double-stranded RNA interference [dsRNAi]) of
OsMAPK5. Interestingly, suppression of OsMAPK5 expression and its kinase
activity resulted in the constitutive expression of pathogenesis-related (PR
) genes such as PR1 and PR10 in the dsRNAi transgenic plants and
significantly enhanced resistance to fungal (Magnaporthe grisea) and bacterial
(Burkholderia glumae) pathogens. However, these same dsRNAi lines had significant
reductions in drought, salt, and cold tolerance. By contrast, overexpression lines
exhibited increased OsMAPK5 kinase activity and increased tolerance to drought, salt,
and cold stresses. These results strongly suggest that OsMAPK5 can positively
regulate drought, salt, and cold tolerance and negatively modulate PR gene
expression and broad-spectrum disease resistance.
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|
|
|
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|
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|
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