Plant Cell Advance Online Publication Published on February 24, 2003; 10.1105/tpc.008714
Received October 20, 2002
Accepted December 21, 2002
Disease Resistance and Abiotic Stress Tolerance in Rice Are Inversely Modulated by
an Abscisic Acid-Inducible Mitogen-Activated Protein Kinase
Lizhong Xiong 1 and Yinong Yang 1*
1
Department of Plant Pathology and Program in Cell and Molecular Biology, University
of Arkansas, Fayetteville, Arkansas 72701
* To whom correspondence should be addressed. E-mail: yiyang{at}uark.edu.
Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating
stress responses in eukaryotic organisms. However, little is known about the role
of MAPKs in modulating the interaction of defense pathways activated by biotic and
abiotic factors. In this study, we have isolated and functionally characterized a
stress-responsive MAPK gene (OsMAPK5) from rice. OsMAPK5 is a single-copy
gene but can generate at least two differentially spliced transcripts. The OsMAPK5
gene, its protein, and kinase activity were inducible by abscisic acid as well
as various biotic (pathogen infection) and abiotic (wounding, drought, salt, and
cold) stresses. To determine its biological function, we generated and analyzed transgenic
rice plants with overexpression (using the 35S promoter of Cauliflower mosaic
virus) or suppression (using double-stranded RNA interference [dsRNAi]) of
OsMAPK5. Interestingly, suppression of OsMAPK5 expression and its kinase
activity resulted in the constitutive expression of pathogenesis-related (PR
) genes such as PR1 and PR10 in the dsRNAi transgenic plants and
significantly enhanced resistance to fungal (Magnaporthe grisea) and bacterial
(Burkholderia glumae) pathogens. However, these same dsRNAi lines had significant
reductions in drought, salt, and cold tolerance. By contrast, overexpression lines
exhibited increased OsMAPK5 kinase activity and increased tolerance to drought, salt,
and cold stresses. These results strongly suggest that OsMAPK5 can positively
regulate drought, salt, and cold tolerance and negatively modulate PR gene
expression and broad-spectrum disease resistance.
This article has been cited by other articles:

|
 |

|
 |
 
L. Chen, F. Ren, H. Zhong, W. Jiang, and X. Li
Identification and expression analysis of genes in response to high-salinity and drought stresses in Brassica napus
Acta Biochim Biophys Sin,
February 1, 2010;
42(2):
154 - 164.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Ning, X. Li, L. M. Hicks, and L. Xiong
A Raf-Like MAPKKK Gene DSM1 Mediates Drought Resistance through Reactive Oxygen Species Scavenging in Rice
Plant Physiology,
February 1, 2010;
152(2):
876 - 890.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. K. You, H. Y. Shin, Y. J. Kim, S. H. Ok, S. K. Cho, J. U. Jeung, S. D. Yoo, J. K. Kim, and J. S. Shin
Novel Bifunctional Nucleases, OmBBD and AtBBD1, Are Involved in Abscisic Acid-Mediated Callose Deposition in Arabidopsis
Plant Physiology,
February 1, 2010;
152(2):
1015 - 1029.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
X. Zhou, P. Cooke, and L. Li
Eukaryotic release factor 1-2 affects Arabidopsis responses to glucose and phytohormones during germination and early seedling development
J. Exp. Bot.,
January 1, 2010;
61(2):
357 - 367.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. Lin, H. Ding, J. Wang, H. Zhang, A. Zhang, Y. Zhang, M. Tan, W. Dong, and M. Jiang
Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling
J. Exp. Bot.,
July 10, 2009;
(2009)
erp157v1.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Q. Ma, X. Dai, Y. Xu, J. Guo, Y. Liu, N. Chen, J. Xiao, D. Zhang, Z. Xu, X. Zhang, et al.
Enhanced Tolerance to Chilling Stress in OsMYB3R-2 Transgenic Rice Is Mediated by Alteration in Cell Cycle and Ectopic Expression of Stress Genes
Plant Physiology,
May 1, 2009;
150(1):
244 - 256.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Mosquera, M. C. Giraldo, C. H. Khang, S. Coughlan, and B. Valent
Interaction Transcriptome Analysis Identifies Magnaporthe oryzae BAS1-4 as Biotrophy-Associated Secreted Proteins in Rice Blast Disease
PLANT CELL,
April 1, 2009;
21(4):
1273 - 1290.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
X. Ding, T. Richter, M. Chen, H. Fujii, Y. S. Seo, M. Xie, X. Zheng, S. Kanrar, R. A. Stevenson, C. Dardick, et al.
A Rice Kinase-Protein Interaction Map
Plant Physiology,
March 1, 2009;
149(3):
1478 - 1492.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Zhang, L.-H. Tian, J.-F. Zhao, Y. Song, C.-J. Zhang, and Y. Guo
Identification of an Apoplastic Protein Involved in the Initial Phase of Salt Stress Response in Rice Root by Two-Dimensional Electrophoresis
Plant Physiology,
February 1, 2009;
149(2):
916 - 928.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B.-Z. Xiao, X. Chen, C.-B. Xiang, N. Tang, Q.-F. Zhang, and L.-Z. Xiong
Evaluation of Seven Function-Known Candidate Genes for their Effects on Improving Drought Resistance of Transgenic Rice under Field Conditions
Mol Plant,
January 1, 2009;
2(1):
73 - 83.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Xu and B. Huang
Root proteomic responses to heat stress in two Agrostis grass species contrasting in heat tolerance
J. Exp. Bot.,
November 13, 2008;
(2008)
ern258v1.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Yasuda, A. Ishikawa, Y. Jikumaru, M. Seki, T. Umezawa, T. Asami, A. Maruyama-Nakashita, T. Kudo, K. Shinozaki, S. Yoshida, et al.
Antagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid-Mediated Abiotic Stress Response in Arabidopsis
PLANT CELL,
June 1, 2008;
20(6):
1678 - 1692.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. J. Rudd, J. Keon, and K. E. Hammond-Kosack
The Wheat Mitogen-Activated Protein Kinases TaMPK3 and TaMPK6 Are Differentially Regulated at Multiple Levels during Compatible Disease Interactions with Mycosphaerella graminicola
Plant Physiology,
June 1, 2008;
147(2):
802 - 815.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Qiu, J. Xiao, W. Xie, H. Liu, X. Li, L. Xiong, and S. Wang
Rice Gene Network Inferred from Expression Profiling of Plants Overexpressing OsWRKY13, a Positive Regulator of Disease Resistance
Mol Plant,
May 1, 2008;
1(3):
538 - 551.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. de Lorenzo, F. Merchan, S. Blanchet, M. Megias, F. Frugier, M. Crespi, and C. Sousa
Differential Expression of the TFIIIA Regulatory Pathway in Response to Salt Stress between Medicago truncatula Genotypes
Plant Physiology,
December 1, 2007;
145(4):
1521 - 1532.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Yara, T. Yaeno, M. Hasegawa, H. Seto, J.-L. Montillet, K. Kusumi, S. Seo, and K. Iba
Disease Resistance against Magnaporthe grisea is Enhanced in Transgenic Rice with Suppression of {omega}-3 Fatty Acid Desaturases
Plant Cell Physiol.,
September 1, 2007;
48(9):
1263 - 1274.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Asselbergh, K. Curvers, S. C. Franca, K. Audenaert, M. Vuylsteke, F. Van Breusegem, and M. Hofte
Resistance to Botrytis cinerea in sitiens, an Abscisic Acid-Deficient Tomato Mutant, Involves Timely Production of Hydrogen Peroxide and Cell Wall Modifications in the Epidermis
Plant Physiology,
August 1, 2007;
144(4):
1863 - 1877.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. Tanabe, K. Yoshimura, A. Kimura, Y. Yabuta, and S. Shigeoka
Differential Expression of Alternatively Spliced mRNAs of Arabidopsis SR Protein Homologs, atSR30 and atSR45a, in Response to Environmental Stress
Plant Cell Physiol.,
July 1, 2007;
48(7):
1036 - 1049.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Xiang, Y. Huang, and L. Xiong
Characterization of Stress-Responsive CIPK Genes in Rice for Stress Tolerance Improvement
Plant Physiology,
July 1, 2007;
144(3):
1416 - 1428.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C.-M. Yeh, P.-S. Chien, and H.-J. Huang
Distinct signalling pathways for induction of MAP kinase activities by cadmium and copper in rice roots
J. Exp. Bot.,
February 1, 2007;
58(3):
659 - 671.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Katou, K. Kuroda, S. Seo, Y. Yanagawa, T. Tsuge, M. Yamazaki, A. Miyao, H. Hirochika, and Y. Ohashi
A Calmodulin-Binding Mitogen-Activated Protein Kinase Phosphatase is Induced by Wounding and Regulates the Activities of Stress-Related Mitogen-Activated Protein Kinases in Rice
Plant Cell Physiol.,
February 1, 2007;
48(2):
332 - 344.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. d. l. M. Dana, J. A. Pintor-Toro, and B. Cubero
Transgenic Tobacco Plants Overexpressing Chitinases of Fungal Origin Show Enhanced Resistance to Biotic and Abiotic Stress Agents
Plant Physiology,
October 1, 2006;
142(2):
722 - 730.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Pitzschke and H. Hirt
Mitogen-Activated Protein Kinases and Reactive Oxygen Species Signaling in Plants
Plant Physiology,
June 1, 2006;
141(2):
351 - 356.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Zhang, M. Jiang, J. Zhang, M. Tan, and X. Hu
Mitogen-Activated Protein Kinase Is Involved in Abscisic Acid-Induced Antioxidant Defense and Acts Downstream of Reactive Oxygen Species Production in Leaves of Maize Plants
Plant Physiology,
June 1, 2006;
141(2):
475 - 487.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Zhang, M. A. R. Mian, and J. H. Bouton
Recent Molecular and Genomic Studies on Stress Tolerance of Forage and Turf Grasses
Crop Sci.,
February 1, 2006;
46(2):
497 - 511.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
X.-C. Yu, M.-J. Li, G.-F. Gao, H.-Z. Feng, X.-Q. Geng, C.-C. Peng, S.-Y. Zhu, X.-J. Wang, Y.-Y. Shen, and D.-P. Zhang
Abscisic Acid Stimulates a Calcium-Dependent Protein Kinase in Grape Berry
Plant Physiology,
February 1, 2006;
140(2):
558 - 579.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Lieberherr, N. P. Thao, A. Nakashima, K. Umemura, T. Kawasaki, and K. Shimamoto
A Sphingolipid Elicitor-Inducible Mitogen-Activated Protein Kinase Is Regulated by the Small GTPase OsRac1 and Heterotrimeric G-Protein in Rice
Plant Physiology,
July 1, 2005;
138(3):
1644 - 1652.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
V. Chinnusamy, A. Jagendorf, and J.-K. Zhu
Understanding and Improving Salt Tolerance in Plants
Crop Sci.,
January 31, 2005;
45(2):
437 - 448.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. P. Anderson, E. Badruzsaufari, P. M. Schenk, J. M. Manners, O. J. Desmond, C. Ehlert, D. J. Maclean, P. R. Ebert, and K. Kazan
Antagonistic Interaction between Abscisic Acid and Jasmonate-Ethylene Signaling Pathways Modulates Defense Gene Expression and Disease Resistance in Arabidopsis
PLANT CELL,
December 1, 2004;
16(12):
3460 - 3479.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C.-M. Yeh, L.-J. Hsiao, and H.-J. Huang
Cadmium Activates a Mitogen-Activated Protein Kinase Gene and MBP Kinases in Rice
Plant Cell Physiol.,
September 15, 2004;
45(9):
1306 - 1312.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Z. Huang and A. Tunnacliffe
Response of human cells to desiccation: comparison with hyperosmotic stress response
J. Physiol.,
July 1, 2004;
558(1):
181 - 191.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Hashimoto, L. Kisseleva, S. Sawa, T. Furukawa, S. Komatsu, and T. Koshiba
A Novel Rice PR10 Protein, RSOsPR10, Specifically Induced in Roots by Biotic and Abiotic Stresses, Possibly via the Jasmonic Acid Signaling Pathway
Plant Cell Physiol.,
May 1, 2004;
45(5):
550 - 559.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
V. Chinnusamy, K. Schumaker, and J.-K. Zhu
Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants
J. Exp. Bot.,
January 2, 2004;
55(395):
225 - 236.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|
|