The relative contributions of cell polarity and nuclear position in specifying the plane of asymmetric division in fucoid zygotes were investigated. In zygotes developing normally, telophase nuclei were positioned parallel to the polar growth axis, and the division plane bisected both axes. To assess division plane specification, the colinearity of the nuclear and growth axes was uncoupled by treatment with pharmacological agents. Spatial correlations between the growth axis, telophase nuclei, and the division plane were analyzed in the treated zygotes. In all cases, cytokinesis was oriented transverse to the telophase mitotic array and was less well aligned with the growth axis. Telophase nuclei also played a predominant role in positioning the division plane in polyspermic zygotes. Microtubules from the telophase nuclei interdigitated throughout the plane of subsequent cytokinesis, and we speculate that they specify the division plane. Morphological markers of the division plane were not observed before telophase; the earliest division marker detected was a plate of actin that assembled in the zone of microtubule overlap late in telophase. These findings are consistent with division plane specification at cytoplast boundaries.