Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on March 4, 2003; 10.1105/tpc.009530

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow All Versions of this Article:
15/4/1034    most recent
tpc.009530v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hao, L.
Right arrow Articles by Bisaro, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hao, L.
Right arrow Articles by Bisaro, D. M.
Agricola
Right arrow Articles by Hao, L.
Right arrow Articles by Bisaro, D. M.

Received December 4, 2002
Accepted January 24, 2003

Geminivirus AL2 and L2 Proteins Interact with and Inactivate SNF1 Kinase

Linhui Hao 1, Hui Wang 1, Garry Sunter 1, and David M. Bisaro 1*

1 Department of Molecular Genetics, Plant Biotechnology Center, and Program in Molecular, Cellular, and Developmental Biology, The Ohio State University, Columbus, Ohio 43210

* To whom correspondence should be addressed. E-mail: bisaro.1{at}osu.edu.

Geminivirus AL2 and L2 proteins cause enhanced susceptibility, characterized primarily by an increase in viral infectivity, when expressed in transgenic plants. Here, we present genetic and biochemical evidence that enhanced susceptibility is attributable to the interaction of AL2 and L2 with SNF1 kinase, a global regulator of metabolism. Specifically, we show that AL2 and L2 inactivate SNF1 in vitro and in vivo. We further demonstrate that expression of an antisense SNF1 transgene in Nicotiana benthamiana plants causes enhanced susceptibility similar to that conditioned by the AL2 and L2 transgenes, whereas SNF1 overexpression leads to enhanced resistance. Transgenic plants expressing an AL2 protein that lacks a significant portion of the SNF1 interaction domain do not display enhanced susceptibility. Together, these observations suggest that the metabolic alterations mediated by SNF1 are a component of innate antiviral defenses and that SNF1 inactivation by AL2 and L2 is a counterdefensive measure. They also indicate that geminiviruses are able to modify host metabolism to their own advantage, and they provide a molecular link between metabolic status and inherent susceptibility to viral pathogens.




This article has been cited by other articles:


Home page
 Mol PlantHome page
E. Baena-Gonzalez
Energy Signaling in the Regulation of Gene Expression during Stress
Mol Plant, January 15, 2010; (2010) ssp113v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Shen, M. I. Reyes, and L. Hanley-Bowdoin
Arabidopsis Protein Kinases GRIK1 and GRIK2 Specifically Activate SnRK1 by Phosphorylating Its Activation Loop
Plant Physiology, June 1, 2009; 150(2): 996 - 1005.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Polge, M. Jossier, P. Crozet, L. Gissot, and M. Thomas
{beta}-Subunits of the SnRK1 Complexes Share a Common Ancestral Function Together with Expression and Function Specificities; Physical Interaction with Nitrate Reductase Specifically Occurs via AKIN{beta}1-Subunit
Plant Physiology, November 1, 2008; 148(3): 1570 - 1582.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. T. Ascencio-Ibanez, R. Sozzani, T.-J. Lee, T.-M. Chu, R. D. Wolfinger, R. Cella, and L. Hanley-Bowdoin
Global Analysis of Arabidopsis Gene Expression Uncovers a Complex Array of Changes Impacting Pathogen Response and Cell Cycle during Geminivirus Infection
Plant Physiology, September 1, 2008; 148(1): 436 - 454.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
X. Yang, S. Baliji, R. C. Buchmann, H. Wang, J. A. Lindbo, G. Sunter, and D. M. Bisaro
Functional Modulation of the Geminivirus AL2 Transcription Factor and Silencing Suppressor by Self-Interaction
J. Virol., November 1, 2007; 81(21): 11972 - 11981.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Shen and L. Hanley-Bowdoin
Geminivirus Infection Up-Regulates the Expression of Two Arabidopsis Protein Kinases Related to Yeast SNF1- and Mammalian AMPK-Activating Kinases
Plant Physiology, December 1, 2006; 142(4): 1642 - 1655.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Gissot, C. Polge, M. Jossier, T. Girin, J.-P. Bouly, M. Kreis, and M. Thomas
AKINbeta{gamma} Contributes to SnRK1 Heterotrimeric Complexes and Interacts with Two Proteins Implicated in Plant Pathogen Resistance through Its KIS/GBD Sequence
Plant Physiology, November 1, 2006; 142(3): 931 - 944.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. H. Florentino, A. A. Santos, M. R. Fontenelle, G. L. Pinheiro, F. M. Zerbini, M. C. Baracat-Pereira, and E. P. B. Fontes
A PERK-Like Receptor Kinase Interacts with the Geminivirus Nuclear Shuttle Protein and Potentiates Viral Infection
J. Virol., July 1, 2006; 80(13): 6648 - 6656.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Wang, K. J. Buckley, X. Yang, R. C. Buchmann, and D. M. Bisaro
Adenosine Kinase Inhibition and Suppression of RNA Silencing by Geminivirus AL2 and L2 Proteins
J. Virol., June 15, 2005; 79(12): 7410 - 7418.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. Trinks, R. Rajeswaran, P. V. Shivaprasad, R. Akbergenov, E. J. Oakeley, K. Veluthambi, T. Hohn, and M. M. Pooggin
Suppression of RNA Silencing by a Geminivirus Nuclear Protein, AC2, Correlates with Transactivation of Host Genes
J. Virol., February 15, 2005; 79(4): 2517 - 2527.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. F. Carvalho and S. G. Lazarowitz
Interaction of the Movement Protein NSP and the Arabidopsis Acetyltransferase AtNSI Is Necessary for Cabbage Leaf Curl Geminivirus Infection and Pathogenicity
J. Virol., October 15, 2004; 78(20): 11161 - 11171.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
E. P.B. Fontes, A. A. Santos, D. F. Luz, A. J. Waclawovsky, and J. Chory
The geminivirus nuclear shuttle protein is a virulence factor that suppresses transmembrane receptor kinase activity
Genes & Dev., October 15, 2004; 18(20): 2545 - 2556.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Vanitharani, P. Chellappan, J. S. Pita, and C. M. Fauquet
Differential Roles of AC2 and AC4 of Cassava Geminiviruses in Mediating Synergism and Suppression of Posttranscriptional Gene Silencing
J. Virol., September 1, 2004; 78(17): 9487 - 9498.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Aminotransferases Confer "Enzymatic Resistance" to Downy Mildew in Melon
PLANT CELL, January 1, 2004; 16(1): 1 - 3.
[Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Viral Defense and Counterdefense: A Role for Adenosine Kinase in Innate Defense and RNA Silencing
PLANT CELL, December 1, 2003; 15(12): 2758 - 2761.
[Full Text] [PDF]

Home page
 Plant CellHome page
H. Wang, L. Hao, C.-Y. Shung, G. Sunter, and D. M. Bisaro
Adenosine Kinase Is Inactivated by Geminivirus AL2 and L2 Proteins
PLANT CELL, December 1, 2003; 15(12): 3020 - 3032.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists