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Plant Cell Advance Online Publication
Published on March 4, 2003; 10.1105/tpc.009845

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Received December 12, 2002
Accepted January 29, 2003

ARC1 Is an E3 Ubiquitin Ligase and Promotes the Ubiquitination of Proteins during the Rejection of Self-Incompatible Brassica Pollen

Sophia L. Stone 1, Erin M. Anderson 2, Robert T. Mullen 2, and Daphne R. Goring 3*

1 Department of Botany, University of Toronto, Toronto, Ontario, Canada M5S 3B2; Biology Department, York University, Toronto, Ontario, Canada M3J 1P3
2 Department of Botany, University of Guelph, Guelph, Ontario, Canada N1G 2W1
3 Department of Botany, University of Toronto, Toronto, Ontario, Canada M5S 3B2

* To whom correspondence should be addressed. E-mail: goring{at}botany.utoronto.ca.

ARC1 is a novel U-box protein required in the Brassica pistil for the rejection of self-incompatible pollen; it functions downstream of the S receptor kinase (SRK). Here, we show that ARC1 has E3 ubiquitin ligase activity and contains several motifs that influence its subcellular localization. ARC1 can shuttle between the nucleus, cytosol, and proteasome/COP9 signalosome (CSN) when expressed in tobacco BY-2 suspension-cultured cells. However, ARC1 localization to the proteasome/CSN occurs only in the presence of an active SRK. In the pistil, ubiquitinated protein levels increase specifically with incompatible pollinations, but they do not change in ARC1 antisense-suppressed pistils. In addition, inhibition of the proteasomal proteolytic activity disrupts the self-incompatibility response. We propose that ARC1 promotes the ubiquitination and proteasomal degradation of compatibility factors in the pistil, which in turn leads to pollen rejection.




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