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Plant Cell Advance Online Publication
Published on May 16, 2003; 10.1105/tpc.010256

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Received December 31, 2002
Accepted March 31, 2003

Role of Nicotianamine in the Intracellular Delivery of Metals and Plant Reproductive Development

Michiko Takahashi 1, Yasuko Terada 2, Izumi Nakai 2, Hiromi Nakanishi 3, Etsuro Yoshimura 3, Satoshi Mori 3, and Naoko K. Nishizawa 4*

1 Laboratory of Plant Biotechnology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2 Department of Applied Chemistry, Science University of Tokyo, Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan
3 Laboratory of Plant Molecular Physiology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
4 Laboratory of Plant Biotechnology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, 332-0012 Saitama, Japan

* To whom correspondence should be addressed. E-mail: annaoko{at}mail.ecc.u-tokyo.ac.jp.

Nicotianamine (NA), a chelator of metals, is ubiquitously present in higher plants. Nicotianamine aminotransferase (NAAT) catalyzes the amino group transfer of NA in the biosynthetic pathway of phytosiderophores and is essential for iron acquisition in graminaceous plants. The gene that encodes NAAT from barley was introduced into the nongraminaceous plant tobacco, which produces NA but not phytosiderophores. Transgenic tobacco plants (naat tobacco) that constitutively expressed the NAAT gene had young leaves with interveinal chlorosis and flowers that were abnormally shaped and sterile. Endogenous NA was consumed as a result of NAAT overproduction in naat tobacco. The resulting NA shortage caused disorders in internal metal transport, leading to these abnormal phenotypes. In addition to its role in long-distance metal transport, NA may be involved in the regulation of metal transfer within the cells. These results suggest that a shortage of NA impaired the functions of metal-requiring proteins, including transcription factors.






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