Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on April 4, 2002; 10.1105/tpc.010422

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow All Versions of this Article:
14/4/805    most recent
tpc.010422v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by He, Y.
Right arrow Articles by Gan, S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by He, Y.
Right arrow Articles by Gan, S.
Agricola
Right arrow Articles by He, Y.
Right arrow Articles by Gan, S.

Received September 27, 2001
Accepted December 21, 2001

A Gene Encoding an Acyl Hydrolase Is Involved in Leaf Senescence in Arabidopsis

Yuehui He 1 and Susheng Gan 1*

1 Plant Physiology/Biochemistry/Molecular Biology Program, Department of Agronomy, and the Tobacco and Health Research Institute, University of Kentucky, Lexington, Kentucky 40546-0236

* To whom correspondence should be addressed. E-mail: sgan{at}uky.edu.

SAG101, a leaf senescence-associated gene, was cloned from an Arabidopsis leaf senescence enhancer trap line and functionally characterized. Reporter gene and RNA gel blot analyses revealed that SAG101 was not expressed until the onset of senescence in leaves. A recombinant SAG101 fusion protein overexpressed in Escherichia coli displayed acyl hydrolase activity. Antisense RNA interference in transgenic plants delayed the onset of leaf senescence for ~4 days. Chemically induced overexpression of SAG101 caused precocious senescence in both attached and detached leaves of transgenic Arabidopsis plants. These data suggest that SAG101 plays a significant role in leaf senescence.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2002 by the American Society of Plant Biologists