Plant Cell Advance Online Publication Published on May 8, 2003; 10.1105/tpc.011965
Received March 14, 2003
Accepted March 24, 2003
Regulation of Expansin Gene Expression Affects Growth and Development in Transgenic
Rice Plants
Dongsu Choi 1, Yi Lee 1, Hyung-Taeg Cho 1, and Hans Kende 2*
1
Michigan State University-Department of Energy Plant Research Laboratory, Michigan
State University, East Lansing, Michigan 48824-1312
2
Michigan State University-Department of Energy Plant Research Laboratory, Michigan
State University, East Lansing, Michigan 48824-1312; Department of Plant Biology,
Michigan State University, East Lansing, Michigan 48824-1312
* To whom correspondence should be addressed. E-mail: hkende{at}msu.edu.
To investigate the in vivo functions of expansins, we generated transgenic rice plants
that express sense and antisense constructs of the expansin gene OsEXP4.
In adult plants with constitutive OsEXP4 expression, 12% of overexpressors
were taller and 88% were shorter than the average control plants, and most overexpressors
developed at least two additional leaves. Antisense plants were shorter and flowered
earlier than the average control plants. In transgenic plants with inducible
OsEXP4 expression, we observed a close correlation between OsEXP4 protein levels
and seedling growth. Coleoptile and mesocotyl length increased by up to 31 and 97%,
respectively, in overexpressors, whereas in antisense seedlings, they decreased by
up to 28 and 43%, respectively. The change in seedling growth resulted from corresponding
changes in cell size, which in turn appeared to be a function of altered cell wall
extensibility. Our results support the hypothesis that expansins are involved in
enhancing growth by mediating cell wall loosening.
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