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Plant Cell Advance Online Publication
Published on August 14, 2003; 10.1105/tpc.012070

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Received March 18, 2003
Accepted July 12, 2003

The Presence of a Chromatin Boundary Appears to Shield a Transgene in Tobacco from RNA Silencing

Ludmila Mlynárová 1, Andrea Hricová 1, Annelies Loonen 2, and Jan-Peter Nap 2*

1 Plant Research International, Wageningen University and Research Centre, NL-6700AA Wageningen, The Netherlands; Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, SK-94901 Nitra, Slovak Republic
2 Plant Research International, Wageningen University and Research Centre, NL-6700AA Wageningen, The Netherlands

* To whom correspondence should be addressed. E-mail: j.p.h.nap{at}plant.wag-ur.nl.

We present isogenic transgenic tobacco lines that carry at a given chromosomal position a {beta}-glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous.






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