Plant Cell Advance Online Publication
Published on September 26, 2003; 10.1105/tpc.014217
Received May 30, 2003
Accepted August 15, 2003
The Novel MYB Protein EPR1 Is a Component of a Slave Circadian Oscillator in Arabidopsis
Norihito Kuno 1, Simon Geir Møller 2*, Tomoko Shinomura 1, XiangMing Xu 3, Nam-Hai Chua 4, and Masaki Furuya 1
1
Hitachi Advanced Research Laboratory, Saitama 350-0395, Japan
2
Laboratory of Plant Molecular Biology, The Rockefeller University, New York, New
York 10021; Department of Biology, University of Leicester, Leicester LE1 7RH,
United Kingdom
3
Department of Biology, University of Leicester, Leicester LE1 7RH, United Kingdom
4
Laboratory of Plant Molecular Biology, The Rockefeller University, New York, New
York 10021
* To whom correspondence should be addressed. E-mail: sgm5{at}le.ac.uk.
Using fluorescent differential display, we identified, from  8000 displayed bands,
a DNA fragment showing rapid induction in response to red light irradiation. This
EARLY-PHYTOCHROME-RESPONSIVE1 gene (EPR1) encodes a novel nucleus-localized
MYB protein harboring a single MYB domain that is highly similar to the circadian
oscillator proteins CCA1 and LHY. EPR1 is regulated by both phytochrome A and phytochrome
B, and the red-light induction of EPR1 is not inhibited by cycloheximide,
demonstrating that EPR1 represents a primary phytochrome-responsive gene.
Our results show that EPR1 overexpression results in enhanced far-red light-induced
cotyledon opening and delayed flowering. In wild-type Arabidopsis plants grown in
continuous light, the EPR1 transcript exhibits circadian rhythmicity similar
to that of CCA1 and LHY. Moreover, EPR1 suppresses its own expression,
suggesting that this protein is part of a regulatory feedback loop. Constitutive
expression of CCA1 and LHY results in the loss of EPR1 rhythmicity, whereas
increased levels of EPR1 have no effect on the central oscillator. We propose
that EPR1 is a component of a slave oscillator that contributes to the refinement
of output pathways, ultimately mediating the correct oscillatory behavior of target
genes.
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