Plant Cell Advance Online Publication
Published on December 5, 2003; 10.1105/tpc.016865
Received September 3, 2003
Accepted October 4, 2003
A CDC45 Homolog in Arabidopsis Is Essential for Meiosis, as Shown by RNA
Interference-Induced Gene Silencing
Rebecca Stevens 1*, Mathilde Grelon 2, Daniel Vezon 2, Jaesung Oh 3, Peter Meyer 3, Claudette Perennes 4, Severine Domenichini 4, and Catherine Bergounioux 4
1
Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique,
Unité Mixte de Recherche 8618, Université de Paris-Sud, 91405 Orsay,
France; Station de Génétique et d'Amélioration des
Plantes, Institut National de la Recherche Agronomique de Versailles, 78026 Versailles,
France
2
Station de Génétique et d'Amélioration des Plantes, Institut
National de la Recherche Agronomique de Versailles, 78026 Versailles, France
3
Leeds Institute for Plant Biotechnology and Agriculture, University of Leeds, Leeds
LS2 9JT, United Kingdom
4
Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique,
Unité Mixte de Recherche 8618, Université de Paris-Sud, 91405 Orsay,
France
* To whom correspondence should be addressed. E-mail: rebecca.stevens{at}avignon.inra.fr.
CDC45 is required for the initiation of DNA replication in yeast and cell
proliferation in mammals and functions as a DNA polymerase  loading factor in
Xenopus. We have cloned a CDC45 homolog from Arabidopsis whose
expression is upregulated at the G1/S transition and in young meiotic flower buds.
One-third of Arabidopsis 35S:CDC45 T1 RNA interference lines are
partially to completely sterile, and the proportion of sterile plants is increased
by using a dmc1 promoter. T1 plants have decreased levels of the CDC45
transcript and contain 21- to 23-bp RNA fragments specific to the CDC45
gene. T2 transgenic lines, in which small RNA fragments are still present,
were used to analyze S-phase entry by 5-bromodeoxyuridine incorporation, which was
not altered compared with that in the wild type. However, microarray data show that
other cell cycle genes are upregulated or downregulated. T2 plants also have highly
reduced fertility. The severity of the phenotype is correlated with the levels of
the CDC45 transcript and small RNA fragments. Severe chromosome fragmentation
arising during meiosis, which is not the result of a defect in the repair of SPO11-induced
Double Strand Breaks, leads to abnormal chromosome segregation and defective pollen
and ovule development.
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