Plant Cell Advance Online Publication
Published on April 14, 2004; 10.1105/tpc.019307
Received November 14, 2003
Accepted March 2, 2004
PHANTASTICA Regulates Development of the Adaxial Mesophyll in Nicotiana Leaves
Neil A. McHale 1 and Ross E. Koning 2
1 Department of Biochemistry and Genetics, Connecticut Agricultural Experiment Station, New Haven, Connecticut 06504
2 Department of Biology, Eastern Connecticut State University, Willimantic, Connecticut 06226
Initiation and growth of leaf blades is oriented by an adaxial/abaxial axis aligned with the original axis of polarity in the leaf primordium. To investigate mechanisms regulating this process, we cloned the Nicotiana tabacum ortholog of PHANTASTICA (NTPHAN) and generated a series of antisense transgenics in N. sylvestris. We show that NSPHAN is expressed throughout emerging blade primordia in the wild type and becomes localized to the middle mesophyll in the expanding lamina. Antisense NSPHAN leaves show ectopic expression of NTH20, a class I KNOX gene. Juvenile transgenic leaves have normal adaxial/abaxial polarity and generate leaf blades in the normal position, but the adaxial mesophyll shows disorganized patterns of cell division, delayed maturation of palisade, and ectopic reinitiation of blade primordia along the midrib. Reversal of the phenotype with exogenous gibberellic acid suggests that NSPHAN, acting via KNOX repression, maintains determinacy in the expanding lamina and sustains the patterns of cell proliferation critical to palisade development.
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