Plant Cell Advance Online Publication
Published on August 17, 2004; 10.1105/tpc.104.023754
Received April 29, 2004
Accepted June 25, 2004
CycD1, a Putative G1 Cyclin from Antirrhinum majus, Accelerates the Cell Cycle in Cultured Tobacco BY-2 Cells by Enhancing Both G1/S Entry and Progression through S and G2 Phases
Olga A. Koroleva 1, Matthew Tomlinson 1, Piyarat Parinyapong 2, Lali Sakvarelidze 1, David Leader 3, Peter Shaw 1, and John H. Doonan 1*
1 John Innes Centre, Norwich, NR4 7UH, United Kingdom
2 School of Biological Sciences, University of Liverpool, Liverpool, L69 3BX, United Kingdom
3 Wheat Improvement Centre-Syngenta, Norwich, NR4 7UH, United Kingdom
* To whom correspondence should be addressed. E-mail: john.doonan{at}bbsrc.ac.uk.
A putative G1 cyclin gene, Antma;CycD1;1 (CycD1), from Antirrhinum majus is known to be expressed throughout the cell cycle in the meristem and other actively proliferating cells. To test its role in cell cycle progression, we examined the effect of CycD1 expression in the tobacco (Nicotiana tabacum) cell suspension culture BY-2. Green fluorescent protein:CycD1 is located in the nucleus throughout interphase. Using epitope-tagged CycD1, we show that it interacts in vivo with CDKA, a cyclin dependent protein kinase that acts at both the G1/S and the G2/M boundaries. We examined the effect of induced expression at different stages of the cell cycle. Expression in G0 cells accelerated entry into both S-phase and mitosis, whereas expression during S-phase accelerated entry into mitosis. Consistent with acceleration of both transitions, the CycD1-associated cyclin dependent kinase can phosphorylate both histone H1 and Rb proteins. The expression of cyclinD1 led to the early activation of total CDK activity, consistent with accelerated cell cycle progression. Continuous expression of CycD1 led to moderate increases in growth rate. Therefore, in contrast with animal D cyclins, CycD1 can promote both G0/G1/S and S/G2/M progression. This indicates that D cyclin function may have diverged between plants and animals.
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