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Plant Cell Advance Online Publication
Published on June 3, 2005; 10.1105/tpc.105.031724

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Received February 11, 2005
Returned for revision May 1, 2005
Accepted May 3, 2005

Transcriptional Profile of the Arabidopsis Root Quiescent Center

Tal Nawy 1, Ji-Young Lee 2, Juliette Colinas 2, Jean Y. Wang 2, Sumena C. Thongrod 3, Jocelyn E. Malamy 4, Kenneth Birnbaum 3, and Philip N. Benfey 2*

1 Department of Biology, Duke University, Durham, North Carolina 27708; Biology Department, New York University, New York, New York 10003
2 Department of Biology, Duke University, Durham, North Carolina 27708
3 Biology Department, New York University, New York, New York 10003
4 Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637

* To whom correspondence should be addressed. E-mail: philip.benfey{at}duke.edu.

The self-renewal characteristics of stem cells render them vital engines of development. To better understand the molecular mechanisms that determine the properties of stem cells, transcript profiling was conducted on quiescent center (QC) cells from the Arabidopsis thaliana root meristem. The AGAMOUS-LIKE 42 (AGL42) gene, which encodes a MADS box transcription factor whose expression is enriched in the QC, was used to mark these cells. RNA was isolated from sorted cells, labeled, and hybridized to Affymetrix microarrays. Comparisons with digital in situ expression profiles of surrounding tissues identified a set of genes enriched in the QC. Promoter regions from a subset of transcription factors identified as enriched in the QC conferred expression in the QC. These studies demonstrated that it is possible to successfully isolate and profile a rare cell type in the plant. Mutations in all enriched transcription factor genes including AGL42 exhibited no detectable root phenotype, raising the possibility of a high degree of functional redundancy in the QC.




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