Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on May 6, 2005; 10.1105/tpc.105.032615

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
17/6/1737    most recent
tpc.105.032615v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chan, J.
Right arrow Articles by Lloyd, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chan, J.
Right arrow Articles by Lloyd, C.
Agricola
Right arrow Articles by Chan, J.
Right arrow Articles by Lloyd, C.

Received March 10, 2005
Returned for revision April 13, 2005
Accepted April 14, 2005

Localization of the Microtubule End Binding Protein EB1 Reveals Alternative Pathways of Spindle Development in Arabidopsis Suspension Cells

Jordi Chan 1*, Grant Calder 1, Samantha Fox 1, and Clive Lloyd 1

1 Department of Cell and Developmental Biology, John Innes Centre, Norwich NR 4 7UH, United Kingdom

* To whom correspondence should be addressed. E-mail: jordi.chan{at}bbsrc.ac.uk.

In a previous study on Arabidopsis thaliana suspension cells transiently infected with the microtubule end binding protein AtEB1a-green fluorescent protein (GFP), we reported that interphase microtubules grow from multiple sites dispersed over the cortex, with plus ends forming the characteristic comet-like pattern. In this study, AtEB1a-GFP was used to study the transitions of microtubule arrays throughout the division cycle of cells lacking a defined centrosome. During division, the dispersed origin of microtubules was replaced by a more focused pattern with the plus end comets growing away from sites associated with the nuclear periphery. The mitotic spindle then evolved in two quite distinct ways depending on the presence or absence of the preprophase band (PPB): the cells displaying outside-in as well as inside-out mitotic pathways. In those cells possessing a PPB, the fusion protein labeled material at the nuclear periphery that segregated into two polar caps, perpendicular to the PPB, before nuclear envelope breakdown (NEBD). These polar caps then marked the spindle poles upon NEBD. However, in the population of cells without PPBs, there was no prepolarization of material at the nuclear envelope before NEBD, and the bipolar spindle only emerged clearly after NEBD. Such cells had variable spindle orientations and enhanced phragmoplast mobility, suggesting that the PPB is involved in a polarization event that promotes early spindle pole morphogenesis and subsequent positional stability during division. Astral-like microtubules are not usually prominent in plant cells, but they are clearly seen in these Arabidopsis cells, and we hypothesize that they may be involved in orienting the division plane, particularly where the plane is not determined before division.




This article has been cited by other articles:


Home page
 J Exp BotHome page
S. A. Oh, M. D. Pal, S. K. Park, J. A. Johnson, and D. Twell
The tobacco MAP215/Dis1-family protein TMBP200 is required for the functional organization of microtubule arrays during male germline establishment
J. Exp. Bot., March 1, 2010; 61(4): 969 - 981.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Komaki, T. Abe, S. Coutuer, D. Inze, E. Russinova, and T. Hashimoto
Nuclear-localized subtype of end-binding 1 protein regulates spindle organization in Arabidopsis
J. Cell Sci., February 1, 2010; 123(3): 451 - 459.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Chan, A. Sambade, G. Calder, and C. Lloyd
Arabidopsis Cortical Microtubules Are Initiated along, as Well as Branching from, Existing Microtubules
PLANT CELL, August 1, 2009; 21(8): 2298 - 2306.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. Bischoff, S. J. Cookson, S. Wu, and W.-R. Scheible
Thaxtomin A affects CESA-complex density, expression of cell wall genes, cell wall composition, and causes ectopic lignification in Arabidopsis thaliana seedlings
J. Exp. Bot., March 1, 2009; 60(3): 955 - 965.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
L. Guo, C.-M. K. Ho, Z. Kong, Y.-R. J. Lee, Q. Qian, and B. Liu
Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome
Ann. Bot., February 1, 2009; 103(3): 387 - 402.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. J. Wright, K. Gallagher, and L. G. Smith
discordia1 and alternative discordia1 Function Redundantly at the Cortical Division Site to Promote Preprophase Band Formation and Orient Division Planes in Maize
PLANT CELL, January 1, 2009; 21(1): 234 - 247.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
J. C. Ambrose and R. Cyr
Mitotic Spindle Organization by the Preprophase Band
Mol Plant, November 1, 2008; 1(6): 950 - 960.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. R. Bisgrove, Y.-R. J. Lee, B. Liu, N. T. Peters, and D. L. Kropf
The Microtubule Plus-End Binding Protein EB1 Functions in Root Responses to Touch and Gravity Signals in Arabidopsis
PLANT CELL, February 1, 2008; 20(2): 396 - 410.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Kirik, U. Herrmann, C. Parupalli, J. C. Sedbrook, D. W. Ehrhardt, and M. Hulskamp
CLASP localizes in two discrete patterns on cortical microtubules and is required for cell morphogenesis and cell division in Arabidopsis
J. Cell Sci., December 15, 2007; 120(24): 4416 - 4425.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Hayashi, T. Sano, N. Kutsuna, F. Kumagai-Sano, and S. Hasezawa
Contribution of Anaphase B to Chromosome Separation in Higher Plant Cells Estimated by Image Processing
Plant Cell Physiol., October 1, 2007; 48(10): 1509 - 1513.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Desprez, M. Juraniec, E. F. Crowell, H. Jouy, Z. Pochylova, F. Parcy, H. Hofte, M. Gonneau, and S. Vernhettes
Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thaliana
PNAS, September 25, 2007; 104(39): 15572 - 15577.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Menand, G. Calder, and L. Dolan
Both chloronemal and caulonemal cells expand by tip growth in the moss Physcomitrella patens
J. Exp. Bot., May 1, 2007; 58(7): 1843 - 1849.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. C. Ambrose and R. Cyr
The Kinesin ATK5 Functions in Early Spindle Assembly in Arabidopsis
PLANT CELL, January 1, 2007; 19(1): 226 - 236.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Dhonukshe, N. Vischer, and T. W. J. Gadella Jr
Contribution of microtubule growth polarity and flux to spindle assembly and functioning in plant cells.
J. Cell Sci., August 1, 2006; 119(Pt 15): 3193 - 3205.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Dixit, E. Chang, and R. Cyr
Establishment of Polarity during Organization of the Acentrosomal Plant Cortical Microtubule Array
Mol. Biol. Cell, March 1, 2006; 17(3): 1298 - 1305.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2005 by the American Society of Plant Biologists