Plant Cell Advance Online Publication
Published on September 9, 2005; 10.1105/tpc.105.035048
Received June 8, 2005
Returned for revision August 15, 2005
Accepted August 22, 2005
Psb29, a Conserved 22-kD Protein, Functions in the Biogenesis of Photosystem II Complexes in Synechocystis and Arabidopsis
Nir Keren 1, Hiroshi Ohkawa 1, Eric A. Welsh 1, Michelle Liberton 1, and Himadri B. Pakrasi 1*
1 Department of Biology, Washington University, St. Louis, Missouri 63130
* To whom correspondence should be addressed. E-mail: pakrasi{at}wustl.edu.
Photosystem II (PSII), the enzyme responsible for photosynthetic oxygen evolution, is a rapidly turned over membrane protein complex. However, the factors that regulate biogenesis of PSII are poorly defined. Previous proteomic analysis of the PSII preparations from the cyanobacterium Synechocystis sp PCC 6803 detected a novel protein, Psb29 (Sll1414), homologs of which are found in all cyanobacteria and vascular plants with sequenced genomes. Deletion of psb29 in Synechocystis 6803 results in slower growth rates under high light intensities, increased light sensitivity, and lower PSII efficiency, without affecting the PSII core electron transfer activities. A T-DNA insertion line in the PSB29 gene in Arabidopsis thaliana displays a phenotype similar to that of the Synechocystis mutant. This plant mutant grows slowly and exhibits variegated leaves, and its PSII activity is light sensitive. Low temperature fluorescence emission spectroscopy of both cyanobacterial and plant mutants shows an increase in the proportion of uncoupled proximal antennae in PSII as a function of increasing growth light intensities. The similar phenotypes observed in both plant and cyanobacterial mutants demonstrate that the function of Psb29 has been conserved throughout the evolution of oxygenic photosynthetic organisms and suggest a role for the Psb29 protein in the biogenesis of PSII.
This article has been cited by other articles:
|
|
|
|
 
T. Wangdi, S. R. Uppalapati, S. Nagaraj, C.-M. Ryu, C. L. Bender, and K. S. Mysore
A Virus-Induced Gene Silencing Screen Identifies a Role for Thylakoid Formation1 in Pseudomonas syringae pv tomato Symptom Development in Tomato and Arabidopsis
Plant Physiology,
January 1, 2010;
152(1):
281 - 292.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Pandelova, M. F. Betts, V. A. Manning, L. J. Wilhelm, T. C. Mockler, and L. M. Ciuffetti
Analysis of Transcriptome Changes Induced by Ptr ToxA in Wheat Provides Insights into the Mechanisms of Plant Susceptibility
Mol Plant,
September 1, 2009;
2(5):
1067 - 1083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ishikawa, M. Fujiwara, K. Sonoike, and N. Sato
Orthogenomics of Photosynthetic Organisms: Bioinformatic and Experimental Analysis of Chloroplast Proteins of Endosymbiont Origin in Arabidopsis and Their Counterparts in Synechocystis
Plant Cell Physiol.,
April 1, 2009;
50(4):
773 - 788.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schottkowski, S. Gkalympoudis, N. Tzekova, C. Stelljes, D. Schunemann, E. Ankele, and J. Nickelsen
Interaction of the Periplasmic PratA Factor and the PsbA (D1) Protein during Biogenesis of Photosystem II in Synechocystis sp. PCC 6803
J. Biol. Chem.,
January 16, 2009;
284(3):
1813 - 1819.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Wegener, E. A. Welsh, L. E. Thornton, N. Keren, J. M. Jacobs, K. K. Hixson, M. E. Monroe, D. G. Camp II, R. D. Smith, and H. B. Pakrasi
High Sensitivity Proteomics Assisted Discovery of a Novel Operon Involved in the Assembly of Photosystem II, a Membrane Protein Complex
J. Biol. Chem.,
October 10, 2008;
283(41):
27829 - 27837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. C. Summerfield and L. A. Sherman
Global Transcriptional Response of the Alkali-Tolerant Cyanobacterium Synechocystis sp. Strain PCC 6803 to a pH 10 Environment
Appl. Envir. Microbiol.,
September 1, 2008;
74(17):
5276 - 5284.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
