Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on June 9, 2006; 10.1105/tpc.105.036269

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
18/7/1722    most recent
tpc.105.036269v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wagner, S.
Right arrow Articles by Hellmann, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wagner, S.
Right arrow Articles by Hellmann, H.
Agricola
Right arrow Articles by Wagner, S.
Right arrow Articles by Hellmann, H.

Received July 16, 2005
Returned for revision April 27, 2006
Accepted May 16, 2006

Analysis of the Arabidopsis rsr4-1/pdx1-3 Mutant Reveals the Critical Function of the PDX1 Protein Family in Metabolism, Development, and Vitamin B6 Biosynthesis

Susan Wagner 1, Anne Bernhardt 1, Jan Erik Leuendorf 1, Christel Drewke 2, Anna Lytovchenko 3, Nader Mujahed 2, Cristian Gurgui 2, Wolf B. Frommer 4, Eckhard Leistner 2, Alisdair R. Fernie 3, and Hanjo Hellmann 1*

1 Angewandte Genetik, Freie Universität Berlin, 14195 Berlin, Germany
2 Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität Bonn, 53115 Bonn, Germany
3 Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14424 Potsdam, Germany
4 Department of Plant Biology, Stanford University, Stanford California 94305

* To whom correspondence should be addressed. E-mail: hellmann{at}zedat.fu-berlin.de.

Vitamin B6 represents a highly important group of compounds ubiquitous in all living organisms. It has been demonstrated to alleviate oxidative stress and in its phosphorylated form participates as a cofactor in >100 biochemical reactions. By means of a genetic approach, we have identified a novel mutant, rsr4-1 (for reduced sugar response), with aberrant root and leaf growth that requires supplementation of vitamin B6 for normal development. Cloning of the mutated gene revealed that rsr4-1 carries a point mutation in a member of the PDX1/SOR1/SNZ (for Pyridoxine biosynthesis protein 1/Singlet oxygen resistant 1/Snooze) family that leads to reduced vitamin B6 content. Consequently, metabolism is broadly altered, mainly affecting amino acid, raffinose, and shikimate contents and trichloroacetic acid cycle constituents. Yeast two-hybrid and pull-down analyses showed that Arabidopsis thaliana PDX1 proteins can form oligomers. Interestingly, the mutant form of PDX1 has severely reduced capability to oligomerize, potentially suggesting that oligomerization is important for function. In summary, our results demonstrate the critical function of the PDX1 protein family for metabolism, whole-plant development, and vitamin B6 biosynthesis in higher plants.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2006 by the American Society of Plant Biologists