Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on April 14, 2006; 10.1105/tpc.105.036376

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow All Versions of this Article:
18/5/1253    most recent
tpc.105.036376v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maruyama, N.
Right arrow Articles by Utsumi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maruyama, N.
Right arrow Articles by Utsumi, S.
Agricola
Right arrow Articles by Maruyama, N.
Right arrow Articles by Utsumi, S.

Received July 21, 2005
Returned for revision January 17, 2006
Accepted March 22, 2006

Multiple Vacuolar Sorting Determinants Exist in Soybean 11S Globulin

Nobuyuki Maruyama 1, Leong Ching Mun 1, Miyuki Tatsuhara 1, Machiko Sawada 1, Masao Ishimoto 2, and Shigeru Utsumi 1*

1 Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan
2 National Agricultural Research Center for Hokkaido Region, Toyohira-ku, Sapporo, Hokkaido 062-8555, Japan

* To whom correspondence should be addressed. E-mail: sutsumi{at}kais.kyoto-u.ac.jp.

The sorting determinants of glycinin, a soybean (Glycine max) 11S globulin, which mediates protein targeting to the protein storage vacuole (PSV), were investigated in maturing soybean cotyledons by transient expression assays. A C-terminal stretch of 10 amino acids of A1aB1b, a glycinin group I subunit, was sufficient to direct green fluorescent protein (GFP) to the PSV. This peptide may correspond to a C-terminal vacuolar sorting determinant (ctVSD). Because functional inhibition of this putative ctVSD of A1aB1b did not block PSV sorting of A1aB1b, we used the three-dimensional structure of A1aB1b to identify candidates for a sequence-specific determinant (ssVSD). We found that the sequence downstream of disordered region 4 could direct GFP to the PSV and that Ile-297 is critical for sorting. However, functional inhibition of the ctVSD, combined with the Ile297Gly mutation, did not abolish the vacuolar sorting of A1aB1b, suggesting that A1aB1b has a third sorting determinant in addition to ctVSD and ssVSD. A glycinin group II subunit, A3B4, lacked a ctVSD but contained a VSD reminiscent of an ssVSD and an additional sorting determinant. We also demonstrate, by expression of dominant negative mutants of small GTPases and drug treatment experiments, that the trafficking of A1aB1b is COPII vesicle-dependent and wortmannin- and brefeldin A-sensitive.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2006 by the American Society of Plant Biologists