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Plant Cell Advance Online Publication
Published on October 14, 2005; 10.1105/tpc.105.036533

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Received July 27, 2005
Returned for revision July 27, 2005
Accepted September 27, 2005

The Arabidopsis STV1 Protein, Responsible for Translation Reinitiation, Is Required for Auxin-Mediated Gynoecium Patterning

Taisuke Nishimura 1, Takuji Wada 2, Kotaro T. Yamamoto 3, and Kiyotaka Okada 4*

1 Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; Plant Science Center, RIKEN, Tsurumi-ku, Yokohama 230-0045, Japan; Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan
2 Plant Science Center, RIKEN, Tsurumi-ku, Yokohama 230-0045, Japan
3 Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan
4 Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; Plant Science Center, RIKEN, Tsurumi-ku, Yokohama 230-0045, Japan; Core Research of Science and Technology Research Project, Kawaguchi, Saitama 322-0012, Japan

* To whom correspondence should be addressed. E-mail: kiyo{at}ok-lab.bot.kyoto-u.ac.jp.

Ribosomal protein L24 (RPL24) is implicated in translation reinitiation of polycistronic genes. A newly isolated Arabidopsis thaliana short valve1 (stv1) mutant, in which one of the RPL24-encoding genes, RPL24B, is deleted, shows specific defects in the apical-basal patterning of the gynoecium, in addition to phenotypes induced by ribosome deficiency. A similar gynoecium phenotype is caused by mutations in the auxin response factor (ARF) genes ETTIN (ETT) and MONOPTEROS (MP), which have upstream open reading frames (uORFs) in their 5'-transcript leader sequences. Gynoecia of a double mutant of stv1 and a weak ett mutant allele are similar to those of a strong ett allele, and transformation with a uORF-eliminated ETT construct partially suppressed the stv1 gynoecium phenotype, implying that STV1 could influence ETT translation through its uORFs. Analyses of 5'-leader-reporter gene fusions showed that the uORFs of ETT and MP negatively regulate the translation of the downstream major ORFs, indicating that translation reinitiation is an important step for the expression of these proteins. Taken together, we propose that perturbation of translation reinitiation of the ARF transcripts causes the defects in gynoecium patterning observed in the stv1 mutant.






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