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Plant Cell Advance Online Publication
Published on February 3, 2006; 10.1105/tpc.105.037978

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Received September 13, 2005
Returned for revision December 30, 2005
Accepted January 9, 2006

Vacuolar H+-ATPase Activity Is Required for Endocytic and Secretory Trafficking in Arabidopsis

Jan Dettmer 1, Anne Hong-Hermesdorf 1, York-Dieter Stierhof 2, and Karin Schumacher 1*

1 Center for Plant Molecular Biology-Plant Physiology, Universität Tübingen, 72076 Tübingen, Germany
2 Center for Plant Molecular Biology-Microscopy, Universität Tübingen, 72076 Tübingen, Germany

* To whom correspondence should be addressed. E-mail: karin.schumacher{at}uni-tuebingen.de.

In eukaryotic cells, compartments of the highly dynamic endomembrane system are acidified to varying degrees by the activity of vacuolar H+-ATPases (V-ATPases). In the Arabidopsis thaliana genome, most V-ATPase subunits are encoded by small gene families, thus offering potential for a multitude of enzyme complexes with different kinetic properties and localizations. We have determined the subcellular localization of the three Arabidopsis isoforms of the membrane-integral V-ATPase subunit VHA-a. Colocalization experiments as well as immunogold labeling showed that VHA-a1 is preferentially found in the trans-Golgi network (TGN), the main sorting compartment of the secretory pathway. Uptake experiments with the endocytic tracer FM4-64 revealed rapid colocalization with VHA-a1, indicating that the TGN may act as an early endosomal compartment. Concanamycin A, a specific V-ATPase inhibitor, blocks the endocytic transport of FM4-64 to the tonoplast, causes the accumulation of FM4-64 together with newly synthesized plasma membrane proteins, and interferes with the formation of brefeldin A compartments. Furthermore, nascent cell plates are rapidly stained by FM4-64, indicating that endocytosed material is redirected into the secretory flow after reaching the TGN. Together, our results suggest the convergence of the early endocytic and secretory trafficking pathways in the TGN.




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