Plant Cell Advance Online Publication
Published on December 9, 2005; 10.1105/tpc.105.038182
Received September 23, 2005
Returned for revision November 8, 2005
Accepted November 14, 2005
Arabidopsis CONSTANS-LIKE3 Is a Positive Regulator of Red Light Signaling and Root Growth
Sourav Datta 1, G.H.C.M. Hettiarachchi 1, Xing-Wang Deng 2, and Magnus Holm 1*
1 Department of Cell and Molecular Biology, Gothenburg University, 405 30 Gothenburg, Sweden
2 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8014
* To whom correspondence should be addressed. E-mail: magnus.holm{at}molbio.gu.se.
CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) is an E3 ubiquitin ligase that represses photomorphogenesis in the dark. Therefore, proteins interacting with COP1 could be important regulators of light-dependent development. Here, we identify CONSTANS-LIKE3 (COL3) as a novel interaction partner of COP1. A green fluorescent protein-COL3 fusion protein colocalizes with COP1 to nuclear speckles when transiently expressed in plant cells. This localization requires the B-box domains in COL3, indicating a novel function of this domain. A loss-of-function col3 mutant has longer hypocotyls in red light and in short days. Unlike constans, the col3 mutant flowers early and shows a reduced number of lateral branches in short days. The mutant also exhibits reduced formation of lateral roots. The col3 mutation partially suppresses the cop1 and deetiolated1 (det1) mutations in the dark, suggesting that COL3 acts downstream of both of these repressors. However, the col3 mutation exerts opposing effects on cop1 and det1 in terms of lateral roots and anthocyanin accumulation, suggesting that COL3 also has activities that are independent of COP1 and DET1. In conclusion, we have identified COL3 as a positive regulator of photomorphogenesis that acts downstream of COP1 but can promote lateral root development independently of COP1 and also function as a daylength-sensitive regulator of shoot branching.
This article has been cited by other articles:
|
|
|
|
 
S. Datta, H. Johansson, C. Hettiarachchi, M. L. Irigoyen, M. Desai, V. Rubio, and M. Holm
LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-Box Protein Involved in Light-Dependent Development and Gene Expression, Undergoes COP1-Mediated Ubiquitination
PLANT CELL,
September 1, 2008;
20(9):
2324 - 2338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Y. P. Chia, A. Muller, C. Jung, and E. S. Mutasa-Gottgens
Sugar beet contains a large CONSTANS-LIKE gene family including a CO homologue that is independent of the early-bolting (B) gene locus
J. Exp. Bot.,
July 1, 2008;
59(10):
2735 - 2748.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Datta, C. Hettiarachchi, H. Johansson, and M. Holm
SALT TOLERANCE HOMOLOG2, a B-Box Protein in Arabidopsis That Activates Transcription and Positively Regulates Light-Mediated Development
PLANT CELL,
October 1, 2007;
19(10):
3242 - 3255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Capoen, J. Den Herder, S. Rombauts, J. De Gussem, A. De Keyser, M. Holsters, and S. Goormachtig
Comparative Transcriptome Analysis Reveals Common and Specific Tags for Root Hair and Crack-Entry Invasion in Sesbania rostrata
Plant Physiology,
August 1, 2007;
144(4):
1878 - 1889.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. M. Al Khateeb and D. F. Schroeder
DDB2, DDB1A and DET1 Exhibit Complex Interactions During Arabidopsis Development
Genetics,
May 1, 2007;
176(1):
231 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Flowering Newsletter bibliography for 2006
J. Exp. Bot.,
April 20, 2007;
(2007)
erm028v2.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Molas, J. Z. Kiss, and M. J. Correll
Gene profiling of the red light signalling pathways in roots
J. Exp. Bot.,
September 1, 2006;
57(12):
3217 - 3229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
