Plant Cell Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on June 9, 2006; 10.1105/tpc.105.038687

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
18/7/1766    most recent
tpc.105.038687v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gaulin, E.
Right arrow Articles by Rickauer, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gaulin, E.
Right arrow Articles by Rickauer, M.
Agricola
Right arrow Articles by Gaulin, E.
Right arrow Articles by Rickauer, M.

Received October 12, 2005
Returned for revision April 21, 2006
Accepted May 11, 2006

Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns

Elodie Gaulin 1*, Nani Dramé 1, Claude Lafitte 1, Trudy Torto-Alalibo 2, Yves Martinez 3, Carine Ameline-Torregrosa 1, Moustafa Khatib 1, Honoré Mazarguil 4, François Villalba-Mateos 1, Sophien Kamoun 2, Christian Mazars 1, Bernard Dumas 1, Arnaud Bottin 1, Marie-Thérèse Esquerré-Tugayé 1, and Martina Rickauer 1

1 Unité Mixte de Recherche 5546, Centre National de la Recherche Scientifique-Université Paul Sabatier-Toulouse III, Pôle de Biotechnologie Végétale, 31326 Castanet-Tolosan, France
2 Ohio State University/Ohio Agricultural Research and Development Center, Department of Plant Pathology, Wooster, Ohio 44691
3 Institut Fédératif de Recherche 40, Pôle de Biotechnologie Végétale, BP42617, 31326 Castanet-Tolosan, France
4 Unité Mixte de Recherche-Centre National de la Recherche Scientifique 5089, Institut de Pharmacologie et de Biologie Structurale, 31077 Toulouse Cedex 04, France

* To whom correspondence should be addressed. E-mail: gaulin{at}scsv.ups-tlse.fr.

The cellulose binding elicitor lectin (CBEL) from Phytophthora parasitica nicotianae contains two cellulose binding domains (CBDs) belonging to the Carbohydrate Binding Module1 family, which is found almost exclusively in fungi. The mechanism by which CBEL is perceived by the host plant remains unknown. The role of CBDs in eliciting activity was investigated using modified versions of the protein produced in Escherichia coli or synthesized in planta through the potato virus X expression system. Recombinant CBEL produced by E. coli elicited necrotic lesions and defense gene expression when injected into tobacco (Nicotiana tabacum) leaves. CBEL production in planta induced necrosis. Site-directed mutagenesis on aromatic amino acid residues located within the CBDs as well as leaf infiltration assays using mutated and truncated recombinant proteins confirmed the importance of intact CBDs to induce defense responses. Tobacco and Arabidopsis thaliana leaf infiltration assays using synthetic peptides showed that the CBDs of CBEL are essential and sufficient to stimulate defense responses. Moreover, CBEL elicits a transient variation of cytosolic calcium levels in tobacco cells but not in protoplasts. These results define CBDs as a novel class of molecular patterns in oomycetes that are targeted by the innate immune system of plants and might act through interaction with the cell wall.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
Y. Brotman, E. Briff, A. Viterbo, and I. Chet
Role of Swollenin, an Expansin-Like Protein from Trichoderma, in Plant Root Colonization
Plant Physiology, June 1, 2008; 147(2): 779 - 789.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. H. Sohn, R. Lei, A. Nemri, and J. D.G. Jones
The Downy Mildew Effector Proteins ATR1 and ATR13 Promote Disease Susceptibility in Arabidopsis thaliana
PLANT CELL, December 1, 2007; 19(12): 4077 - 4090.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2006 by the American Society of Plant Biologists