Plant Cell Advance Online Publication
Published on March 3, 2006; 10.1105/tpc.105.039636
Received November 22, 2005
Returned for revision January 19, 2006
Accepted February 7, 2006
The D-Type Cyclin CYCD3;1 Is Limiting for the G1-to-S-Phase Transition in Arabidopsis
Margit Menges 1, Anne K. Samland 1, Séverine Planchais 1, and James A.H. Murray 1*
1 Institute of Biotechnology, University of Cambridge, CB2 1QT Cambridge, United Kingdom
* To whom correspondence should be addressed. E-mail: j.murray{at}biotech.cam.ac.uk.
The G1-to-S-phase transition is a key regulatory point in the cell cycle, but the rate-limiting component in plants is unknown. Overexpression of CYCLIN D3;1 (CYCD3;1) in transgenic plants increases mitotic cycles and reduces endocycles, but its effects on cell cycle progression cannot be unambiguously determined. To analyze the cell cycle roles of plant D-type cyclins, we overexpressed CYCD3;1 in Arabidopsis thaliana cell suspension cultures. Changes in cell number and doubling time were insignificant, but cultures exhibited an increased proportion of G2- over G1-phase cells, as well as increased G2 arrest in response to stationary phase and sucrose starvation. Synchronized cultures confirm that CYCD3;1-expressing (but not CYCD2;1-expressing) cells show increased G2-phase length and delayed activation of mitotic genes such as B-type cyclins, suggesting that CYCD3;1 has a specific G1/S role. Analysis of putative cyclin-dependent kinase phosphorylation sites within CYCD3;1 shows that mutating Ser-343 to Ala enhances CYCD3;1 potency without affecting its rate of turnover and results in a fivefold increase in the level of cell death in response to sucrose removal. We conclude that CYCD3;1 dominantly drives the G1/S transition, and in sucrose-depleted cells the decline in CYCD3;1 levels leads to G1 arrest, which is overcome by ectopic CYCD3;1 expression. Ser-343 is likely a key residue in modulating CYCD3;1 activity in response to sucrose depletion.
This article has been cited by other articles:
|
|
|
|
 
I. A. Paponov, M. Paponov, W. Teale, M. Menges, S. Chakrabortee, J. A.H. Murray, and K. Palme
Comprehensive Transcriptome Analysis of Auxin Responses in Arabidopsis
Mol Plant,
March 1, 2008;
1(2):
321 - 337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Menges, G. Pavesi, P. Morandini, L. Bogre, and J. A.H. Murray
Genomic Organization and Evolutionary Conservation of Plant D-Type Cyclins
Plant Physiology,
December 1, 2007;
145(4):
1558 - 1576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Dewitte, S. Scofield, A. A. Alcasabas, S. C. Maughan, M. Menges, N. Braun, C. Collins, J. Nieuwland, E. Prinsen, V. Sundaresan, et al.
Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses
PNAS,
September 4, 2007;
104(36):
14537 - 14542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peres, M. L. Churchman, S. Hariharan, K. Himanen, A. Verkest, K. Vandepoele, Z. Magyar, Y. Hatzfeld, E. Van Der Schueren, G. T. S. Beemster, et al.
Novel Plant-specific Cyclin-dependent Kinase Inhibitors Induced by Biotic and Abiotic Stresses
J. Biol. Chem.,
August 31, 2007;
282(35):
25588 - 25596.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, C. Smith, F. Corke, L. Zheng, Z. Merali, P. Ryden, P. Derbyshire, K. Waldron, and M. W. Bevan
Signaling from an Altered Cell Wall to the Nucleus Mediates Sugar-Responsive Growth and Development in Arabidopsis thaliana
PLANT CELL,
August 1, 2007;
19(8):
2500 - 2515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kono, C. Umeda-Hara, S. Adachi, N. Nagata, M. Konomi, T. Nakagawa, H. Uchimiya, and M. Umeda
The Arabidopsis D-Type Cyclin CYCD4 Controls Cell Division in the Stomatal Lineage of the Hypocotyl Epidermis
PLANT CELL,
April 1, 2007;
19(4):
1265 - 1277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Churchman, M. L. Brown, N. Kato, V. Kirik, M. Hulskamp, D. Inze, L. De Veylder, J. D. Walker, Z. Zheng, D. G. Oppenheimer, et al.
SIAMESE, a Plant-Specific Cell Cycle Regulator, Controls Endoreplication Onset in Arabidopsis thaliana
PLANT CELL,
November 1, 2006;
18(11):
3145 - 3157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
