Plant Cell Advance Online Publication
Published on June 9, 2006; 10.1105/tpc.105.039677
OPEN ACCESS ARTICLE
Received November 23, 2005
Returned for revision April 29, 2006
Accepted May 11, 2006
A Comprehensive Structure-Function Analysis of Arabidopsis SNI1 Defines Essential Regions and Transcriptional Repressor Activity
Rebecca A. Mosher 1, Wendy E. Durrant 1, Dong Wang 1, Junqi Song 1, and Xinnian Dong 1*
1 Developmental, Cell, and Molecular Biology Group, Department of Biology, Duke University, Durham, North Carolina 27708
* To whom correspondence should be addressed. E-mail: xdong{at}duke.edu.
The expression of systemic acquired resistance (SAR) in plants involves the upregulation of many Pathogenesis-Related (PR) genes, which work in concert to confer resistance to a broad spectrum of pathogens. Because SAR is a costly process, SAR-associated transcription must be tightly regulated. Arabidopsis thaliana SNI1 (for Suppressor of NPR1, Inducible) is a negative regulator of SAR required to dampen the basal expression of PR genes. Whole genome transcriptional profiling showed that in the sni1 mutant, Nonexpresser of PR genes (NPR1)-dependent benzothiadiazole S-methylester-responsive genes were specifically derepressed. Interestingly, SNI1 also repressed transcription when expressed in yeast, suggesting that it functions as an active transcriptional repressor through a highly conserved mechanism. Chromatin immunoprecipitation indicated that histone modification may be involved in SNI1-mediated repression. Sequence comparison with orthologs in other plant species and a saturating NAAIRS-scanning mutagenesis of SNI1 identified regions in SNI1 that are required for its activity. The structural similarity of SNI1 to Armadillo repeat proteins implies that SNI1 may form a scaffold for interaction with proteins that modulate transcription.
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