Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on December 22, 2006; 10.1105/tpc.106.040840

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
18/12/3370    most recent
tpc.106.040840v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mizoi, J.
Right arrow Articles by Nishida, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mizoi, J.
Right arrow Articles by Nishida, I.
Agricola
Right arrow Articles by Mizoi, J.
Right arrow Articles by Nishida, I.

Received January 4, 2006
Returned for revision October 31, 2006
Accepted November 10, 2006

Defects in CTP:PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE Affect Embryonic and Postembryonic Development in Arabidopsis

Junya Mizoi 1, Masanobu Nakamura 1, and Ikuo Nishida 2*

1 Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
2 Department of Life Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama City, Saitama 338-8570, Japan

* To whom correspondence should be addressed. E-mail: nishida{at}molbiol.saitama-u.ac.jp.

A TILLING strategy (for targeting-induced local-scale lesions in genomes) was used in Arabidopsis thaliana to isolate mutants of a gene encoding CTP:PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE (PECT; EC 2.7.7.14), a rate-limiting enzyme in phosphatidylethanolamine biosynthesis. A null mutation, pect1-6, caused embryo abortion before the octant stage. However, reciprocal crosses revealed that pect1-6 caused no significant gametophytic defect. In pect1-4, PECT activity was decreased by 74%. Growth was generally normal in these mutants, despite delays in embryo maturation and reduced fertility. At low temperatures, however, homozygotic pect1-4 plants displayed dwarfism. PECT activity was decreased by 47% in heterozygotic pect1-6 plants and by 80% in pect1-4/pect1-6 F1 plants, which also displayed a small but significant decrease of phosphatidylethanolamine and a reciprocal increase in phosphatidylcholine. These lipid changes were fully reversed by wild-type PECT1 expression. pect1-4/pect1-6 F1 plants displayed severe dwarfism, tissue abnormalities, and low fertility, which was attributable in part to inhibition of anther, embryo, and ovule development, as was the reduced fertility of pect1-4 seedlings. PECT1 cDNA expression under the control of an inducible promoter partially rectified the mutant phenotypes observed in pect1-4/pect1-6 F1 seedlings, indicating that malfunctions in different tissues have a synergistic effect on the mutant phenotypes.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Nerlich, M. von Orlow, D. Rontein, A. D. Hanson, and P. Dormann
Deficiency in Phosphatidylserine Decarboxylase Activity in the psd1 psd2 psd3 Triple Mutant of Arabidopsis Affects Phosphatidylethanolamine Accumulation in Mitochondria
Plant Physiology, June 1, 2007; 144(2): 904 - 914.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2006 by the American Society of Plant Biologists