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Plant Cell Advance Online Publication
Published on December 22, 2006; 10.1105/tpc.106.040923

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Received January 6, 2006
Returned for revision October 5, 2006
Accepted November 10, 2006

Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins

Daniël Van Damme 1, Silvie Coutuer 1, Riet De Rycke 1, Francois-Yves Bouget 1, Dirk Inzé 1, and Danny Geelen 1*

1 Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnologie, Ghent University, B-9052 Gent, Belgium

* To whom correspondence should be addressed. E-mail: danny.geelen{at}ugent.be.

TPLATE was previously identified as a potential cytokinesis protein targeted to the cell plate. Disruption of TPLATE in Arabidopsis thaliana leads to the production of shriveled pollen unable to germinate. Vesicular compartmentalization of the mature pollen is dramatically altered, and large callose deposits accumulate near the intine cell wall layer. Green fluorescent protein (GFP)-tagged TPLATE expression under the control of the pollen promoter Lat52 complements the phenotype. Downregulation of TPLATE in Arabidopsis seedlings and tobacco (Nicotiana tabacum) BY-2 suspension cells results in crooked cell walls and cell plates that fail to insert into the mother wall. Besides accumulating at the cell plate, GFP-fused TPLATE is temporally targeted to a narrow zone at the cell cortex where the cell plate connects to the mother wall. TPLATE-GFP also localizes to subcellular structures that accumulate at the pollen tube exit site in germinating pollen. Ectopic callose depositions observed in mutant pollen also occur in RNA interference plants, suggesting that TPLATE is implicated in cell wall modification. TPLATE contains domains similar to adaptin and {beta}-COP coat proteins. These data suggest that TPLATE functions in vesicle-trafficking events required for site-specific cell wall modifications during pollen germination and for anchoring of the cell plate to the mother wall at the correct cortical position.




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