Plant Cell Advance Online Publication
Published on May 5, 2006; 10.1105/tpc.106.041640
Received February 1, 2006
Returned for revision March 17, 2006
Accepted March 30, 2006
The Arabidopsis Major Intrinsic Protein NIP5;1 Is Essential for Efficient Boron Uptake and Plant Development under Boron Limitation
Junpei Takano 1, Motoko Wada 1, Uwe Ludewig 2, Gabriel Schaaf 3, Nicolaus von Wirén 3, and Toru Fujiwara 4*
1 Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2 Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universität Tübingen, 72076 Tübingen, Germany
3 Institut für Pflanzenernährung, Universität Hohenheim, D-70593 Stuttgart, Germany
4 Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Solution Oriented Research for Science and Technology, Japan Science and Technology Agency, Chuo-Ku, Tokyo 103-0027, Japan
* To whom correspondence should be addressed. E-mail: atorufu{at}mail.ecc.u-tokyo.ac.jp.
Boron (B) is essential in plants but often present at low concentrations in the environment. To investigate how plants survive under conditions of B limitation, we conducted a transcriptome analysis and identified NIP5;1, a member of the major intrinsic protein family, as a gene upregulated in B-deficient roots of Arabidopsis thaliana. Promoter- -glucuronidase fusions indicated that NIP5;1 is strongly upregulated in the root elongation zone and the root hair zone under B limitation, and green fluorescent protein-tagged NIP5;1 proteins localized to the plasma membrane. Expression in Xenopus laevis oocytes demonstrated that NIP5;1 facilitated the transport of boric acid in addition to water. Importantly, two T-DNA insertion lines of NIP5;1 displayed lower boric acid uptake into roots, lower biomass production, and increased sensitivity of root and shoot development to B deficiency. These results identify NIP5;1 as a major plasma membrane boric acid channel crucial for the B uptake required for plant growth and development under B limitation.
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