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Plant Cell Advance Online Publication
Published on April 13, 2007; 10.1105/tpc.106.042895

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Received April 1, 2006
Returned for revision March 8, 2007
Accepted March 22, 2007

The Nuclear-Encoded Factor HCF173 Is Involved in the Initiation of Translation of the psbA mRNA in Arabidopsis thaliana

Kerstin Schult 1, Karin Meierhoff 1*, Susanne Paradies 1, Thomas Töller 2, Petra Wolff 2, and Peter Westhoff 1

1 Institut für Entwicklungs und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany
2 Institut für Physikalische Biologie, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany

* To whom correspondence should be addressed. E-mail: karin.meierhoff{at}uni-duesseldorf.de.

To gain insight into the biogenesis of photosystem II (PSII) and to identify auxiliary factors required for this process, we characterized the mutant hcf173 of Arabidopsis thaliana. The mutant shows a high chlorophyll fluorescence phenotype (hcf) and is severely affected in the accumulation of PSII subunits. In vivo labeling experiments revealed a drastically decreased synthesis of the reaction center protein D1. Polysome association experiments suggest that this is primarily caused by reduced translation initiation of the corresponding psbA mRNA. Comparison of mRNA steady state levels indicated that the psbA mRNA is significantly reduced in hcf173. Furthermore, the determination of the psbA mRNA half-life revealed an impaired RNA stability. The HCF173 gene was identified by map-based cloning, and its identity was confirmed by complementation of the hcf phenotype. HCF173 encodes a protein with weak similarities to the superfamily of the short-chain dehydrogenases/reductases. The protein HCF173 is localized in the chloroplast, where it is mainly associated with the membrane system and is part of a higher molecular weight complex. Affinity chromatography of an HCF173 fusion protein uncovered the psbA mRNA as a component of this complex.




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C. Schwarz, I. Elles, J. Kortmann, M. Piotrowski, and J. Nickelsen
Synthesis of the D2 Protein of Photosystem II in Chlamydomonas Is Controlled by a High Molecular Mass Complex Containing the RNA Stabilization Factor Nac2 and the Translational Activator RBP40
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