Plant Cell Advance Online Publication
Published on September 22, 2006; 10.1105/tpc.106.044230
Received May 18, 2006
Returned for revision July 28, 2006
Accepted August 30, 2006
An Arabidopsis Glutathione Peroxidase Functions as Both a Redox Transducer and a Scavenger in Abscisic Acid and Drought Stress Responses
Yuchen Miao 1, Dong Lv 2, Pengcheng Wang 2, Xue-Chen Wang 3, Jia Chen 3, Chen Miao 2, and Chun-Peng Song 2*
1 Henan Key Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng 475001, China; State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
2 Henan Key Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng 475001, China
3 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
* To whom correspondence should be addressed. E-mail: songcp{at}henu.edu.cn.
We isolated two T-DNA insertion mutants of Arabidopsis thaliana GLUTATHIONE PEROXIDASE3 (ATGPX3) that exhibited a higher rate of water loss under drought stress, higher sensitivity to H2O2 treatment during seed germination and seedling development, and enhanced production of H2O2 in guard cells. By contrast, lines engineered to overexpress ATGPX3 were less sensitive to drought stress than the wild type and displayed less transpirational water loss, which resulted in higher leaf surface temperature. The atgpx3 mutation also disrupted abscisic acid (ABA) activation of calcium channels and the expression of ABA- and stress-responsive genes. ATGPX3 physically interacted with the 2C-type protein phosphatase ABA INSENSITIVE2 (ABI2) and, to a lesser extent, with ABI1. In addition, the redox states of both ATGPX3 and ABI2 were found to be regulated by H2O2. The phosphatase activity of ABI2, measured in vitro, was reduced approximately fivefold by the addition of oxidized ATGPX3. The reduced form of ABI2 was converted to the oxidized form by the addition of oxidized ATGPX3 in vitro, which might mediate ABA and oxidative signaling. These results suggest that ATGPX3 might play dual and distinctive roles in H2O2 homeostasis, acting as a general scavenger and specifically relaying the H2O2 signal as an oxidative signal transducer in ABA and drought stress signaling.
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