Plant Cell Advance Online Publication
Published on February 16, 2007; 10.1105/tpc.106.044792
OPEN ACCESS ARTICLE
Received June 10, 2006
Returned for revision December 21, 2006
Accepted January 31, 2007
TCP Transcription Factors Control the Morphology of Shoot Lateral Organs via Negative Regulation of the Expression of Boundary-Specific Genes in Arabidopsis
Tomotsugu Koyama 1, Masahiko Furutani 2, Masao Tasaka 2, and Masaru Ohme-Takagi 1*
1 Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012, Japan
2 Nara Institute of Science and Technology, Graduate School of Biological Sciences, Ikoma, Nara 630-0101, Japan
* To whom correspondence should be addressed. E-mail: m-takagi{at}aist.go.jp.
Plants form shoot meristems in the so-called boundary region, and these meristems are necessary for normal morphogenesis of aerial parts of plants. However, the molecular mechanisms that regulate the formation of shoot meristems are not fully understood. We report here that expression of a chimeric repressor from TCP3 (TCP3SRDX), a member of TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) transcription factors in Arabidopsis thaliana, resulted in the formation of ectopic shoots on cotyledons and various defects in organ development. Expression of TCP3SRDX induced ectopic expression of boundary-specific genes, namely the CUP-SHAPED COTYLEDON (CUC) genes, and suppressed the expression of miR164, whose product cleaves the transcripts of CUC genes. This abnormal phenotype was substantially reversed on the cuc1 mutant background. By contrast, gain of function of TCP3 suppressed the expression of CUC genes and resulted in the fusion of cotyledons and defects in formation of shoots. The pattern of expression of TCP3 did not overlap with that of the CUC genes. In addition, we found that eight TCPs had functions similar to that of TCP3. Our results demonstrate that the TCP transcription factors play a pivotal role in the control of morphogenesis of shoot organs by negatively regulating the expression of boundary-specific genes.
This article has been cited by other articles:
|
|
|
|
 
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2007
J. Exp. Bot.,
July 18, 2008;
(2008)
ern109v1.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Broholm, S. Tahtiharju, R. A. E. Laitinen, V. A. Albert, T. H. Teeri, and P. Elomaa
A TCP domain transcription factor controls flower type specification along the radial axis of the Gerbera (Asteraceae) inflorescence
PNAS,
July 1, 2008;
105(26):
9117 - 9122.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Heyl, E. Ramireddy, W. G. Brenner, M. Riefler, J. Allemeersch, and T. Schmulling
The Transcriptional Repressor ARR1-SRDX Suppresses Pleiotropic Cytokinin Activities in Arabidopsis
Plant Physiology,
July 1, 2008;
147(3):
1380 - 1395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Lopez-Juez, E. Dillon, Z. Magyar, S. Khan, S. Hazeldine, S. M. de Jager, J. A.H. Murray, G. T.S. Beemster, L. Bogre, and H. Shanahan
Distinct Light-Initiated Gene Expression and Cell Cycle Programs in the Shoot Apex and Cotyledons of Arabidopsis
PLANT CELL,
April 1, 2008;
20(4):
947 - 968.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
